@article{fdi:010068261,
  title = {{M}orphological, molecular and {MALDI}-{TOF} mass spectrometry identification of ixodid tick species collected in {O}romia, {E}thiopia},
  author = {{K}umsa, {B}. and {L}aroche, {M}. and {A}lmeras, {L}. and {M}ediannikov, {O}leg and {R}aoult, {D}idier and {P}arola, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{M}atrix-assisted laser desorption/ionization time-of-flight mass spectrometry ({MALDI}-{TOF} {MS}) technology has recently been reported as a promising method for arthropods identification. {M}ore recently, our laboratory reported the correct identification of tick species via the {MALDI}-{TOF} {MS} protein spectra profiling of legs from fresh specimens. {T}he aim of the present study was to assess the use of {MALDI}-{TOF} {MS} for correct identification of ixodid tick species preserved in 70 % ethanol during field collection in {E}thiopia. {F}ollowing morphological identification of 12 tick species, the legs from 85 tick specimens were subjected to {MALDI}-{TOF} {MS}. {S}pectral analysis revealed an intra-species reproducibility and inter-species specificity that were consistent with the morphological classification. {T}o support the results of the {MALDI}-{TOF} {MS} tick species identification, 41 tick specimens comprising 3 to 5 specimens per tick species were used to create a reference spectra database, which was evaluated using the spectra of the 44 remaining tick specimens. {T}he blind tests revealed that 100 % of the tick specimens studied by {MALDI}-{TOF} {MS} were correctly identified. {A} relevant {L}og score value ({LSV}) of > 1.8 was recorded for all of the tick species studied by {MALDI}-{TOF} {MS}, except for {R}hipicephalus praetextatus. {T}he morphological and {MALDI}-{TOF} {MS} identifications were confirmed by sequencing the 12{S} ribosomal {RNA} (r{RNA}) gene of 40 tick specimens belonging to 11 ixodid species. {T}aken together, the results of the present study indicate that {MALDI}-{TOF} {MS} is a reliable tool for tick species identification, even after preservation in ethanol, provided that a reference spectra database is built from specimens that represent the respective species stored under the same conditions.},
  keywords = {{T}icks ; {M}orphology ; {MALDI}-{TOF}-{MS} ; {E}thanol ; {O}romia ; {E}thiopia ; {ETHIOPIE}},
  booktitle = {},
  journal = {{P}arasitology {R}esearch},
  volume = {115},
  numero = {11},
  pages = {4199--4210},
  ISSN = {0932-0113},
  year = {2016},
  DOI = {10.1007/s00436-016-5197-9},
  URL = {https://www.documentation.ird.fr/hor/fdi:010068261},
}