@article{fdi:010068224,
  title = {{E}nhanced transfer of biologically fixed {N} from faba bean to intercropped wheat through mycorrhizal symbiosis},
  author = {{W}ahbi, {S}. and {M}aghraoui, {T}. and {H}afidi, {M}. and {S}anguin, {H}. and {O}ufdou, {K}. and {P}rin, {Y}. and {D}uponnois, {R}obin and {G}aliana, {A}.},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n {M}orocco, the use of seed legumes is limited because significant water deficits and the low availability of soil phosphorus ({P}) limit nitrogen fixation. {H}owever, little is known about the ability of faba bean-rhizobium symbiosis to fix nitrogen in {P}-deficient soils and to transfer fixed nitrogen ({N}) to intercropped wheat. {A}rbuscular mycorrhizal fungi ({AM}) and their extraradical hyphae networks play an important role in the facilitation process by promoting interconnectivity and the transfer of nutrients, such as {N} and {P}, between associated plants. {T}he aim of this study was to analyse the impact of {AM} inoculation on {N}-2 fixation and the transfer of fixed {N} from faba bean to intercropped wheat. {G}erminated faba bean and wheat seeds were transferred into 1-l pots filled with a {P}-deficient soil that was collected from the {H}aouz valley near {M}arrakech ({M}orocco). {P}lants from the two species were grown in pots in either pure or mixed stands under greenhouse conditions, and each cropping system was subjected to three mycorrhizal inoculation treatments with a non-inoculated ({AM}0) and two concentrations of {R}hizophagus irregularis inoculants containing 1000 ({AM}1) or 2000 ({AM}2) spores pot(-1). {T}he {N}-15 isotope dilution method was used to determine the amount and proportion of atmospheric {N} fixed by faba bean ({N}dfa%) and the fixed {N} that was transferred to wheat. {M}ycorrhizal inoculation had a significantly positive effect on the shoot dry weights and total shoot {N} in faba bean, but not in wheat. {T}he cropping system had no significant effect on the plant growth and total shoot {N} in both faba bean and wheat. {T}he {N}dfa percentage was very high in all of the treatments, varying from 86 to 91%. {T}he total {N} fixed by faba bean was 27% significantly higher in the {AM}2 treatment compared with the {AM}1 and {AM}0 treatments for both cropping systems combined. {T}he estimated proportions of fixed {N} that were transferred from faba bean to wheat were far higher in {AM}1 (50%) and {AM}2 (32%) treatments than in {AM}0 (15%) treatment as well as for the total transferred fixed {N}. {A}s corroborated by a parallel observation of root mycorrhizal colonization, these results suggest that the development of mycorrhizal networks stimulates the transfer of fixed {N} from faba bean to wheat, which could significantly contribute to the facilitation process under intercropping conditions.},
  keywords = {{A}rbuscular mycorrhizal fungi ; {F}acilitation ; {N}-15 isotopic dilution ; {I}ntercropping systems ; {L}ow-input agricultural management ; {N}itrogen fixation ; {MAROC}},
  booktitle = {},
  journal = {{A}pplied {S}oil {E}cology},
  volume = {107},
  numero = {},
  pages = {91--98},
  ISSN = {0929-1393},
  year = {2016},
  DOI = {10.1016/j.apsoil.2016.05.008},
  URL = {https://www.documentation.ird.fr/hor/fdi:010068224},
}