@article{fdi:010068173,
  title = {{D}iazotroph derived nitrogen supports diatom growth in the {S}outh {W}est {P}acific : a quantitative study using nano{SIMS}},
  author = {{B}onnet, {S}ophie and {B}erthelot, {H}. and {T}urk-{K}ubo, {K}. and {C}ornet-{B}arthaux, {V}. and {F}awcett, {S}. and {B}erman-{F}rank, {I}. and {B}arani, {A}. and {G}regori, {G}. and {D}ekaezemacker, {J}. and {B}enavides, {M}. and {C}apone, {D}. {G}.},
  editor = {},
  language = {{ENG}},
  abstract = {{N}itrogen is essential for life but is often a major limiting nutrient for growth in the ocean. {B}iological dinitrogen fixation is a major source of new nitrogen to surface waters and promotes marine productivity. {Y}et the fate of diazotroph-derived nitrogen ({DDN}) in marine ecosystems has been poorly studied, and its transfer to auto- and heterotrophic plankton has not been measured. {H}ere, we use high-resolution nanometer scale secondary ion mass spectrometry (nano{SIMS}) coupled with {N}-15(2) isotopic labelling and flow cytometry cell sorting to examine the {DDN} transfer to specific groups of natural phytoplankton and bacteria during three diazotroph blooms dominated by the cyanobacterium {T}richodesmium spp. in the {S}outh {W}est {P}acific. {D}uring these experiments, 13%+/- 2% to 48%+/- 5% of the fixed {N}-15(2) was released into the dissolved pool and 6%+/- 1% to 8%+/- 2% of this {DDN} was transferred to non-diazotrophic plankton after 48 h. {T}he primary beneficiaries of this {DDN} were diatoms (45%+/- 4% to 61%+/- 38%) and bacteria (22%+/- 27% to 38%+/- 12%), followed by pico-phytoplankton (3%+/- 1% to 21%+/- 14%). {T}he {DDN} was quickly converted to non-diazotrophic plankton biomass, in particular that of diatoms, which increased in abundance by a factor of 1.4-15 over the course of the three experiments. {T}he single-cell approach we used enabled quantification of the actual transfer of {DDN} to specific groups of autotrophic and heterotrophic plankton in the surface ocean, revealing a previously unseen level of complexity in the pathways that occur between {N}-2 fixation and the eventual export of {DDN} from the photic zone.},
  keywords = {{PACIFIQUE} {SUD} {OUEST} ; {NOUVELLE} {CALEDONIE} ; {SALOMON} {MER}},
  booktitle = {},
  journal = {{L}imnology and {O}ceanography},
  volume = {61},
  numero = {5},
  pages = {1549--1562},
  ISSN = {0024-3590},
  year = {2016},
  DOI = {10.1002/lno.10300},
  URL = {https://www.documentation.ird.fr/hor/fdi:010068173},
}