@article{fdi:010068156,
  title = {{P}araburkholderia nodosa is the main {N}-2-fixing species trapped by promiscuous common bean ({P}haseolus vulgaris {L}.) in the {B}razilian '{C}erradao'},
  author = {{D}all'{A}gnol, {R}. {F}. and {P}lotegher, {F}. and {S}ouza, {R}. {C}. and {M}endes, {I}. {C}. and dos {R}eis, {F}. {B}. and {B}{\'e}na, {G}illes and {M}oulin, {L}ionel and {H}ungria, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he bacterial genus {B}urkholderia comprises species occupying several habitats, including a group of symbionts of leguminous plants-also called beta-rhizobia-that has been recently ascribed to the new genus {P}araburkholderia. {W}e used common bean ({P}haseolus vulgaris {L}.) plants to trap rhizobia from an undisturbed soil of the {B}razilian {C}errado under the vegetation type '{C}erradao'. {G}enetic characterization started with the analyses of 181 isolates by {BOX}-{PCR}, where the majority revealed unique profiles, indicating high inter- and intra-species diversity. {R}estriction fragment length polymorphism-{PCR} of the 16{S} r{RNA} of representative strains of the {BOX}-{PCR} groups indicated two main clusters, and gene-sequencing analysis identified the minority (27%) as {R}hizobium and the majority (73%) as {P}araburkholderia. {P}hylogenetic analyses of the 16{S} r{RNA} and housekeeping (rec{A} and gyr{B}) genes positioned all strains of the second cluster in the species {P}. nodosa, and the phylogeny of a symbiotic gene-nod{C}-was in agreement with the conserved genes. {A}ll isolates were stable vis-{A} -vis nodulating common bean, but, in general, with a low capacity for fixing {N}-2, although some effective strains were identified. {T}he predominance of {P}. nodosa might be associated with the edaphic properties of the {C}errado biome, and might represent an important role in terms of maintenance of the ecosystem, which is characterized by acid soils with high saturation of aluminum and low {N}-2 content.{S}tudy of the diversity of nitrogen-fixing symbionts from a 'hot spot area', the {B}razilian '{C}erradao', reveals that {P}araburkholderia nodosa is the main symbiont trapped by promiscuous common bean ({P}haseolus vulgaris {L}.).{S}tudy of the diversity of nitrogen-fixing symbionts from a 'hot spot area', the {B}razilian '{C}erradao', reveals that {P}araburkholderia nodosa is the main symbiont trapped by promiscuous common bean ({P}haseolus vulgaris {L}.).},
  keywords = {beta-rhizobia ; phylogeny of prokaryotes ; biological {N}-2 fixation ; {C}errado ; {BRESIL}},
  booktitle = {},
  journal = {{F}ems {M}icrobiology {E}cology},
  volume = {92},
  numero = {8},
  pages = {fiw108 [14 p.]},
  ISSN = {0168-6496},
  year = {2016},
  DOI = {10.1093/femsec/fiw108},
  URL = {https://www.documentation.ird.fr/hor/fdi:010068156},
}