@article{fdi:010068101,
  title = {{M}olecular analysis of human {P}apillomavirus detected among women positive for cervical lesions by visual inspection with acetic acid/{L}ugol's iodine ({VIA}/{VILI}) in {L}ibreville, {G}abon},
  author = {{M}oussavou, {P}. {B}. and {K}oumakpayi, {I}. {H}. and {N}kili-{M}eyong, {A}. {A}. and {L}abouba, {I}. and {B}isvigou, {U}. and {C}hansi, {J}. {K}. and {E}ngohan-{A}loghe, {C}. and {D}issanami, {F}. and {A}mbounda, {N}. and {D}elannoy-{V}ieillard, {A}. {S}. and {D}iancourt, {L}. and {N}koghe, {D}. and {L}eroy, {E}ric and {B}elembaogo, {E}. and {B}erthet, {N}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {T}he human papillomavirus ({HPV}) is the causative agent of cervical cancer, which is the leading cancer-related cause of death for women in {S}ub-{S}aharan {A}frica. {I}n 2013, the {G}abonese {M}inistry of {H}ealth and the {S}ylvia {B}ongo {O}ndimba {F}oundation implemented cervical cancer screening programs based on the detection of cancerous lesions by visual inspection with acetic acid and/or {L}ugol's iodine ({VIA}/{VILI}). {T}his pilot study was set up to determine the {HPV} profile and analyze the nucleotide sequence variation of {HPV}16 circulating in patients with cervical abnormalities detected by {VIA}/{VILI} testing. {M}ethods: {T}he cervical abnormalities observed upon {VIA}/{VILI} were confirmed by liquid-based cytology for all tested women. {N}ested {PCR} using the {MY}09/11 and {GP}5+/6+ primer sets was used to detect {HPV}s present in the extracted {DNA}. {HPV} genotypes were determined after sequencing of amplicons based on a high-throughput sequencing approach. {F}or isolates of the {HPV}16 genotype, the {E}6 gene and the long control region ({LCR}) were directly sequenced using {S}anger method. {R}esults: {T}he study included 87 women who showed a positive {VIA}/{VILI} result. {C}ervical abnormalities were found in 40.23 % of women and 40 % were classified as high-grade lesions. {T}he {HPV} detection rate was 82.9 % among women with abnormal cytology. {A}mong all the identified high-risk {HPV} genotypes, {HPV}16, 18 and 33 were the most frequent. {M}ultiple {HPV} infections were observed in 42.31 % of {HPV}-infected women. {A}nalysis of the {HPV}16 sequence variation in the {E}6 gene and in the {LCR} showed that 85.3 and 14.7 % belonged to the {A}frican and {E}uropean lineages, respectively. {A}mong the {A}frican branch variants, {A}f2 was the most frequently identified in this study. {C}onclusion: {T}his study offers the first report of the {HPV} detection rate and molecular epidemiology among {G}abonese women with a positive result in a {VIA}/{VILI} screening test. {M}oreover, data on the {HPV}16 sequence variation confirm the predominance of {A}frican variants in high-grade lesions.},
  keywords = {{H}uman papillomavirus ; {D}etection rate ; {V}isual inspection ; {M}olecular variant ; {G}abon ; {GABON}},
  booktitle = {},
  journal = {{I}nfectious {A}gents and {C}ancer},
  volume = {11},
  numero = {},
  pages = {art. 50 [9 p.]},
  ISSN = {1750-9378},
  year = {2016},
  DOI = {10.1186/s13027-016-0098-1},
  URL = {https://www.documentation.ird.fr/hor/fdi:010068101},
}