@article{fdi:010067787,
  title = {{C}omparison of the performances of five primer sets for the detection and quantification of {P}lasmodium in anopheline vectors by real-time {PCR}},
  author = {{C}haumeau, {V}. and {A}ndolina, {C}. and {F}ustec, {B}{\'e}n{\'e}dicte and {N}dam, {N}. {T}. and {B}rengues, {C}{\'e}cile and {H}erder, {S}t{\'e}phane and {C}erqueira, {D}. and {C}hareonviriyaphap, {T}. and {N}osten, {F}. and {C}orbel, {V}incent},
  editor = {},
  language = {{ENG}},
  abstract = {{Q}uantitative real-time polymerase chain reaction (qrt{PCR}) has made a significant improvement for the detection of {P}lasmodium in anopheline vectors. {A} wide variety of primers has been used in different assays, mostly adapted from molecular diagnosis of malaria in human. {H}owever, such an adaptation can impact the sensitivity of the {PCR}. {T}herefore we compared the sensitivity of five primer sets with different molecular targets on blood stages, sporozoites and oocysts standards of {P}lasmodium falciparum ({P}f) and {P}. vivax ({P}v). {D}ilution series of standard {DNA} were used to discriminate between methods at low concentrations of parasite and to generate standard curves suitable for the absolute quantification of {P}lasmodium sporozoites. {O}ur results showed that the best primers to detect blood stages were not necessarily the best ones to detect sporozoites. {A}bsolute detection threshold of our qrt{PCR} assay varied between 3.6 and 360 {P}v sporozoites and between 6 and 600 {P}f sporozoites per mosquito according to the primer set used in the reaction mix. {I}n this paper, we discuss the general performance of each primer set and highlight the need to use efficient detection methods for transmission studies.},
  keywords = {},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {11},
  numero = {7},
  pages = {e0159160 [15 p.]},
  ISSN = {1932-6203},
  year = {2016},
  DOI = {10.1371/journal.pone.0159160},
  URL = {https://www.documentation.ird.fr/hor/fdi:010067787},
}