Publications des scientifiques de l'IRD

Chaumeau V., Andolina C., Fustec Bénédicte, Ndam N. T., Brengues Cécile, Herder Stéphane, Cerqueira D., Chareonviriyaphap T., Nosten F., Corbel Vincent. (2016). Comparison of the performances of five primer sets for the detection and quantification of Plasmodium in anopheline vectors by real-time PCR. Plos One, 11 (7), p. e0159160 [15 p.]. ISSN 1932-6203.

Titre du document
Comparison of the performances of five primer sets for the detection and quantification of Plasmodium in anopheline vectors by real-time PCR
Année de publication
2016
Type de document
Article référencé dans le Web of Science WOS:000380797500037
Auteurs
Chaumeau V., Andolina C., Fustec Bénédicte, Ndam N. T., Brengues Cécile, Herder Stéphane, Cerqueira D., Chareonviriyaphap T., Nosten F., Corbel Vincent
Source
Plos One, 2016, 11 (7), p. e0159160 [15 p.] ISSN 1932-6203
Quantitative real-time polymerase chain reaction (qrtPCR) has made a significant improvement for the detection of Plasmodium in anopheline vectors. A wide variety of primers has been used in different assays, mostly adapted from molecular diagnosis of malaria in human. However, such an adaptation can impact the sensitivity of the PCR. Therefore we compared the sensitivity of five primer sets with different molecular targets on blood stages, sporozoites and oocysts standards of Plasmodium falciparum (Pf) and P. vivax (Pv). Dilution series of standard DNA were used to discriminate between methods at low concentrations of parasite and to generate standard curves suitable for the absolute quantification of Plasmodium sporozoites. Our results showed that the best primers to detect blood stages were not necessarily the best ones to detect sporozoites. Absolute detection threshold of our qrtPCR assay varied between 3.6 and 360 Pv sporozoites and between 6 and 600 Pf sporozoites per mosquito according to the primer set used in the reaction mix. In this paper, we discuss the general performance of each primer set and highlight the need to use efficient detection methods for transmission studies.
Plan de classement
Entomologie médicale / Parasitologie / Virologie [052]
Localisation
Fonds IRD [F B010067787]
Identifiant IRD
fdi:010067787
Contact