@article{fdi:010067783,
  title = {{B}iotechnological strategies for studying actinorhizal symbiosis in {C}asuarinaceae : transgenesis and beyond},
  author = {{F}roussart, {E}milie and {Z}hong, {C}. {L}. and {J}iang, {Q}. {B}. and {B}onneau, {J}ocelyne and {B}ogusz, {D}idier and {F}ranche, {C}laudine},
  editor = {},
  language = {{ENG}},
  abstract = {{S}ince the recovery of the first transgenic plant in the early 1980s, plant transformation technologies have enabled advances in many aspects of basic plant science, including nitrogen-fixing root endosymbioses. {U}sing the biological vectors {A}grobacterium tumefaciens and {A}. rhizogenes, gene constructs have been successfully introduced in the actinorhizal tree species {C}asuarina glauca, thereby paving the way for functional analysis of the key genes involved in the symbiotic process with the actinobacterium {F}rankia. {I}n recent years, not only studies of gene promoters in transgenic {C}asuarinaceae, but also the use of {RNA} interference to down-regulate genes of interest, have provided new insights into the early stages of the interaction between the root system and the actinobacterium. {O}pportunities offered by recent developments in genome editing technologies based on the engineered nucleases {ZFN}s (zinc-finger nucleases), {TALEN}s (transcription activator-like effector nucleases) and {RNA}-guided {CRISPR}-{C}as (clustered regularly interspaced short palindromic repeats-associated protein/{C}as) will be briefly presented.},
  keywords = {{C}asuarina ; {N}itrogen fixation ; {A}ctinorhizal nodule ; {A}grobacterium ; {G}enome editing},
  booktitle = {},
  journal = {{S}ymbiosis},
  volume = {70},
  numero = {1},
  pages = {101--109},
  ISSN = {0334-5114},
  year = {2016},
  DOI = {10.1007/s13199-016-0400-4},
  URL = {https://www.documentation.ird.fr/hor/fdi:010067783},
}