@article{fdi:010067778,
  title = {{M}etagenomic and {PCR}-based diversity surveys of [{F}e{F}e]-hydrogenases combined with isolation of alkaliphilic hydrogen-producing bacteria from the serpentinite-hosted {P}rony hydrothermal field, {N}ew {C}aledonia},
  author = {{M}ei, {N}. and {P}ostec, {A}. and {M}onnin, {C}. and {P}elletier, {B}ernard and {P}ayri, {C}laude and {M}enez, {B}. and {F}rouin, {E}. and {O}llivier, {B}ernard and {E}rauso, {G}. and {Q}u{\'e}m{\'e}neur, {M}arianne},
  editor = {},
  language = {{ENG}},
  abstract = {{H}igh amounts of hydrogen are emitted in the serpentinite-hosted hydrothermal field of the {P}rony {B}ay ({PHF}, {N}ew {C}aledonia), where high-p{H} (similar to 11), low-temperature (< 40 degrees {C}), and low-salinity fluids are discharged in both intertidal and shallow submarine environments. {I}n this study, we investigated the diversity and distribution of potentially hydrogen-producing bacteria in {P}rony hyperalkaline springs by using metagenomic analyses and different {PCR}-amplified {DNA} sequencing methods. {T}he retrieved sequences of hyd{A} genes, encoding the catalytic subunit of [{F}e{F}e]-hydrogenases and, used as a molecular marker of hydrogen-producing bacteria, were mainly related to those of {F}irmicutes and clustered into two distinct groups depending on sampling locations. {I}ntertidal samples were dominated by new hyd{A} sequences related to uncultured {F}irmicutes retrieved from paddy soils, while submarine samples were dominated by diverse hyd{A} sequences affiliated with anaerobic and/or thermophilic submarine {F}irmicutes pertaining to the orders {T}herrnoanaerobacterales or {C}lostridiales. {T}he novelty and diversity of these [{F}e{F}e]-hydrogenases may reflect the unique environmental conditions prevailing in the {PHF} (i.e., high-p{H}, low-salt, mesothermic fluids). {I}n addition, novel alkaliphilic hydrogen-producing {F}irmicutes ({C}lostridiales and {B}acillales) were successfully isolated from both intertidal and submarine {PHF} chimney samples. {B}oth molecular and cultivation-based data demonstrated the ability of {F}irmicutes originating from serpentinite-hosted environments to produce hydrogen by fermentation, potentially contributing to the molecular hydrogen balance in situ.},
  keywords = {hydrogen ; microbial diversity ; hydrogen producers ; serpentinization ; hyd{A} genes ; [{F}e{F}e]-hydrogenase ; metagenomics ; {NOUVELLE} {CALEDONIE} ; {PACIFIQUE}},
  booktitle = {},
  journal = {{F}rontiers in {M}icrobiology},
  volume = {7},
  numero = {},
  pages = {art. 1301 [17 p.]},
  ISSN = {1664-302{X}},
  year = {2016},
  DOI = {10.3389/fmicb.2016.01301},
  URL = {https://www.documentation.ird.fr/hor/fdi:010067778},
}