@article{fdi:010067728,
  title = {{C}alculation of the {ELISA}'s cut-off based on the change-point analysis method for detection of {T}rypanosoma cruzi infection in {B}olivian dogs in the absence of controls},
  author = {{L}ardeux, {F}r{\'e}d{\'e}ric and {T}orrico, {G}. and {A}liaga, {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{I}n {ELISA}s, sera of individuals infected by {T}rypanosoma cruzi show absorbance values above a cut-off value. {T}he cut-off is generally computed by means of formulas that need absorbance readings of negative (and sometimes positive) controls, which are included in the titer plates amongst the unknown samples. {W}hen no controls are available, other techniques should be employed such as change-point analysis. {T}he method was applied to {B}olivian dog sera processed by {ELISA} to diagnose {T}. cruzi infection. {I}n each titer plate, the change-point analysis estimated a step point which correctly discriminated among known positive and known negative sera, unlike some of the six usual cut-off formulas tested. {T}o analyse the {ELISA}s results, the change-point method was as good as the usual cut-off formula of the form "mean + 3 standard deviation of negative controls". {C}hange-point analysis is therefore an efficient alternative method to analyse {ELISA} absorbance values when no controls are available.},
  keywords = {{ELISA} ; cut-off ; change-point analysis ; {T}rypanosoma cruzi ; dog ; {B}olivia ; {BOLIVIE} ; {LA} {PAZ}},
  booktitle = {},
  journal = {{M}emorias do {I}nstituto {O}swaldo {C}ruz},
  volume = {111},
  numero = {8},
  pages = {501--504},
  ISSN = {0074-0276},
  year = {2016},
  DOI = {10.1590/0074-02760160119},
  URL = {https://www.documentation.ird.fr/hor/fdi:010067728},
}