@article{fdi:010067711,
  title = {{L}ack of artemisinin resistance in {P}lasmodium falciparum in northwest {B}enin after 10 years of use of artemisinin-based combination therapy},
  author = {{O}gouyemi-{H}ounto, {A}. and {D}amien, {G}. and {D}eme, {A}. {B}. and {T}uikue {N}dam, {N}icaise and {A}ssohou, {C}. and {T}chonlin, {D}. and {M}ama, {A}. and {H}ounkpe, {V}. {O}. and {M}outouama, {J}. {D}. and {R}emou{\'e}, {F}ranck and {N}diaye, {D}. and {G}azard, {D}. {K}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A}im: {I}n {B}enin, artemisinin-based combination therapy ({ACT}) has been recommended as the first-line treatment for uncomplicated {P}lasmodium falciparum malaria since 2004. {T}he emergence in {S}outheast {A}sia of parasites that are resistant to artemisinins poses a serious threat to global control of this disease. {T}he presence of artemisinin resistance genotypes in parasite populations in {B}enin is currently unknown. {T}he present study investigated the prevalence of relevant {K}13-propeller gene polymorphisms in parasite isolates from the north-western region of {B}enin. {M}ethod: {P}lasmodium falciparum isolates were collected from children with a confirmed diagnosis of malaria aged 6 months to 5 years in two towns, {C}obly and {D}jougou, in the north-western part of {B}enin. {T}he study was conducted during the rainy season from {J}uly to {N}ovember 2014 in local health facilities. {T}he {K}13-propeller gene was amplified in parasite isolates using nested {PCR} and subsequently sequenced. {R}esults: {A} total of 108 children were recruited into the study. {T}he efficiency of amplification reactions was 72% (78/108). {T}he propeller domain of the {K}13 gene was successfully sequenced in 78 {P}. falciparum isolates; all of them were wild type with no polymorphisms detectable. {C}onclusion: {T}he absence of mutations in the {K}13 gene indicates that {P}. falciparum parasite populations in the study area are still fully susceptible to artemisinins.},
  keywords = {{P}lasmodium falciparum ; {M}alaria ; {R}esistance ; {A}rtemisinin ; {K}13 propeller ; {B}enin ; {BENIN}},
  booktitle = {},
  journal = {{P}arasite},
  volume = {23},
  numero = {},
  pages = {art. 28 [6 p.]},
  ISSN = {1252-607{X}},
  year = {2016},
  DOI = {10.1051/parasite/2016028},
  URL = {https://www.documentation.ird.fr/hor/fdi:010067711},
}