@article{fdi:010067694,
  title = {{D}ual {RNA}-seq reveals {M}eloidogyne graminicola transcriptome and candidate effectors during the interaction with rice plants},
  author = {{P}etitot, {A}nne-{S}ophie and {D}ereeper, {A}lexis and {A}gbessi, {M}. and {D}a {S}ilva, {C}. and {G}uy, {J}. and {A}rdisson, {M}. and {F}ernandez, {D}iana},
  editor = {},
  language = {{ENG}},
  abstract = {{R}oot-knot nematodes secrete proteinaceous effectors into plant tissues to facilitate infection by suppressing host defences and reprogramming the host metabolism to their benefit. {M}eloidogyne graminicola is a major pest of rice ({O}ryza sativa) in {A}sia and {L}atin {A}merica, causing important crop losses. {T}he goal of this study was to identify {M}. graminicola pathogenicity genes expressed during the plant-nematode interaction. {U}sing the dual {RNA}-sequencing ({RNA}-seq) strategy, we generated transcriptomic data of {M}. graminicola samples covering the pre-parasitic {J}2 stage and five parasitic stages in rice plants, from the parasitic {J}2 to the adult female. {I}n the absence of a reference genome, a de novo {M}. graminicola transcriptome of 66 396 contigs was obtained from those reads that were not mapped on the rice genome. {G}ene expression profiling across the {M}. graminicola life cycle revealed key genes involved in nematode development and provided insights into the genes putatively associated with parasitism. {T}he development of a secreted protein prediction' pipeline revealed a typical set of proteins secreted by nematodes, as well as a large number of cysteine-rich proteins and putative nuclear proteins. {C}ombined with expression data, this pipeline enabled the identification of 15 putative effector genes, including two homologues of well-characterized effectors from cyst nematodes ({CLE}-like and {VAP}1) and a metallothionein. {T}he localization of gene expression was assessed by in situ hybridization for a subset of candidates. {A}ll of these data represent important molecular resources for the elucidation of {M}. graminicola biology and for the selection of potential targets for the development of novel control strategies for this nematode species.},
  keywords = {dual {RNA}-seq ; effector genes ; expression profiling ; {M}eloidogyne graminicola ; {O}ryza sativa ; plant-parasitic nematode},
  booktitle = {},
  journal = {{M}olecular {P}lant {P}athology},
  volume = {17},
  numero = {6},
  pages = {860--874},
  ISSN = {1464-6722},
  year = {2016},
  DOI = {10.1111/mpp.12334},
  URL = {https://www.documentation.ird.fr/hor/fdi:010067694},
}