@article{fdi:010067673,
  title = {{E}ucalyptus hairy roots, a fast, efficient and versatile tool to explore function and expression of genes involved in wood formation},
  author = {{P}lasencia, {A}. and {S}oler, {M}. and {D}upas, {A}. and {L}adouce, {N}. and {S}ilva-{M}artins, {G}. and {M}artinez, {Y}. and {L}apierre, {C}. and {F}ranche, {C}laudine and {T}ruchet, {I}. and {G}rima-{P}ettenati, {J}.},
  editor = {},
  language = {{ENG}},
  abstract = {{E}ucalyptus are of tremendous economic importance being the most planted hardwoods worldwide for pulp and paper, timber and bioenergy. {T}he recent release of the {E}ucalyptus grandis genome sequence pointed out many new candidate genes potentially involved in secondary growth, wood formation or lineage-specific biosynthetic pathways. {T}heir functional characterization is, however, hindered by the tedious, time-consuming and inefficient transformation systems available hitherto for eucalypts. {T}o overcome this limitation, we developed a fast, reliable and efficient protocol to obtain and easily detect co-transformed {E}. grandis hairy roots using fluorescent markers, with an average efficiency of 62%. {W}e set up conditions both to cultivate excised roots in vitro and to harden composite plants and verified that hairy root morphology and vascular system anatomy were similar to wild-type ones. {W}e further demonstrated that co-transformed hairy roots are suitable for medium-throughput functional studies enabling, for instance, protein subcellular localization, gene expression patterns through {RT}-q{PCR} and promoter expression, as well as the modulation of endogenous gene expression. {D}own-regulation of the {E}ucalyptus cinnamoyl-{C}o{A} reductase1 ({E}g{CCR}1) gene, encoding a key enzyme in lignin biosynthesis, led to transgenic roots with reduced lignin levels and thinner cell walls. {T}his gene was used as a proof of concept to demonstrate that the function of genes involved in secondary cell wall biosynthesis and wood formation can be elucidated in transgenic hairy roots using histochemical, transcriptomic and biochemical approaches. {T}he method described here is timely because it will accelerate gene mining of the genome for both basic research and industry purposes.},
  keywords = {{A}grobacterium rhizogenes ; {E}ucalyptus ; hairy roots ; xylem ; secondary cell ; wall ; lignin},
  booktitle = {},
  journal = {{P}lant {B}iotechnology {J}ournal},
  volume = {14},
  numero = {6},
  pages = {1381--1393},
  ISSN = {1467-7644},
  year = {2016},
  DOI = {10.1111/pbi.12502},
  URL = {https://www.documentation.ird.fr/hor/fdi:010067673},
}