@article{fdi:010067671,
  title = {{A}n epidemiologically successful {E}scherichia coli sequence type modulates {P}lasmodium falciparum infection in the mosquito midgut},
  author = {{T}chioffo, {M}. {T}. and {A}bate, {L}uc and {B}oissi{\`e}re, {A}nne and {N}sango, {S}. {E}. and {G}imonneau, {G}eoffrey and {B}erry, {A}. and {O}swald, {E}. and {D}ubois, {D}. and {M}orlais, {I}sabelle},
  editor = {},
  language = {{ENG}},
  abstract = {{M}alaria transmission relies on the successful development of {P}lasmodium parasites in the {A}nopheles mosquito vector. {W}ithin the mosquito midgut, malaria parasites encounter a resident bacterial flora and parasite-bacteria interactions modulate {P}lasmodium development. {T}he mechanisms by which the bacteria interact with malaria parasites are still unknown. {T}he intestinal microbiota could regulate immune signaling pathways or produce bacterial compounds that block {P}lasmodium development. {I}n this study, we characterized {E}scherichia coli strains previously isolated from the {A}nopheles mosquito midgut and investigated the putative role of two {E}. coli clones, 444({ST}95) and 351({ST}73), on parasite development. {S}porogonic development was significantly impacted by exposure to clone 444({ST}95) whereas prevalence and intensity of infection were not different in mosquitoes challenged with 351({ST}73) as compared to control mosquitoes. {T}his result indicates midgut bacteria exhibit intra-specific variation in their ability to inhibit {P}lasmodium development. {E}xpression patterns of immune genes differed between mosquitoes challenged with 444({ST}95) and 351({ST}73) and examination of the luminal midgut surface by transmission electron microscopy revealed distinct effects of bacterial exposure on midgut epithelial cells. {T}he 444({ST}95) clone strongly affected mosquito survival and parasite development and this could be associated to the {H}emolysin {F} or other toxins released by the bacteria. {F}urther studies will be needed to decipher the virulence factors and to determine their contribution to the observed phenotype of the 444({ST}95) {E}. coli strain that belongs to the epidemiological {ST}95 clonal group responsible for extra intestinal infections in human and other animals.},
  keywords = {{P}lasmodium falciparum ; {P}arasite inhibition ; {I}ntra-specific variation ; {E}scherichia coli ; {S}equence type ({ST}95) ; {CAMEROUN}},
  booktitle = {},
  journal = {{I}nfection {G}enetics and {E}volution},
  volume = {43},
  numero = {},
  pages = {22--30},
  ISSN = {1567-1348},
  year = {2016},
  DOI = {10.1016/j.meegid.2016.05.002},
  URL = {https://www.documentation.ird.fr/hor/fdi:010067671},
}