%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Lopez, D.
%A Ben Amira, M.
%A Brown, D.
%A Muries, B.
%A Brunel-Michac, N.
%A Bourgerie, S.
%A Porcheron, B.
%A Lemoine, R.
%A Chrestin, Hervé
%A Mollison, E.
%A Di Cola, A.
%A Frigerio, L.
%A Julien, J. L.
%A Gousset-Dupont, A.
%A Fumanal, B.
%A Label, P.
%A Pujade-Renaud, V.
%A Auguin, D.
%A Venisse, J. S.
%T The Hevea brasiliensis XIP aquaporin subfamily : genomic, structural and functional characterizations with relevance to intensive latex harvesting
%D 2016
%L fdi:010067659
%G ENG
%J Plant Molecular Biology
%@ 0167-4412
%K XIP aquaporin ; Hevea brasiliensis ; Latex ; Evolution ; Glycerol ; Cell homeostasis
%M ISI:000379037800001
%N 4-5
%P 375-396
%R 10.1007/s11103-016-0462-y
%U https://www.documentation.ird.fr/hor/fdi:010067659
%> https://www.documentation.ird.fr/intranet/publi/2016/08/010067659.pdf
%V 91
%W Horizon (IRD)
%X X-Intrinsic Proteins (XIP) were recently identified in a narrow range of plants as a full clade within the aquaporins. These channels reportedly facilitate the transport of a wide range of hydrophobic solutes. The functional roles of XIP in planta remain poorly identified. In this study, we found three XIP genes (HbXIP1;1, HbXIP2;1 and HbXIP3;1) in the Hevea brasiliensis genome. Comprehensive bioinformatics, biochemical and structural analyses were used to acquire a better understanding of this AQP subfamily. Phylogenetic analysis revealed that HbXIPs clustered into two major groups, each distributed in a specific lineage of the order Malpighiales. Tissue-specific expression profiles showed that only HbXIP2;1 was expressed in all the vegetative tissues tested (leaves, stem, bark, xylem and latex), suggesting that HbXIP2;1 could take part in a wide range of cellular processes. This is particularly relevant to the rubber-producing laticiferous system, where this isoform was found to be up-regulated during tapping and ethylene treatments. Furthermore, the XIP transcriptional pattern is significantly correlated to latex production level. Structural comparison with SoPIP2;1 from Spinacia oleracea species provides new insights into the possible role of structural checkpoints by which HbXIP2;1 ensures glycerol transfer across the membrane. From these results, we discuss the physiological involvement of glycerol and HbXIP2;1 in water homeostasis and carbon stream of challenged laticifers. The characterization of HbXIP2;1 during rubber tree tapping lends new insights into molecular and physiological response processes of laticifer metabolism in the context of latex exploitation.
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