Publications des scientifiques de l'IRD

Lopez D., Ben Amira M., Brown D., Muries B., Brunel-Michac N., Bourgerie S., Porcheron B., Lemoine R., Chrestin Hervé, Mollison E., Di Cola A., Frigerio L., Julien J. L., Gousset-Dupont A., Fumanal B., Label P., Pujade-Renaud V., Auguin D., Venisse J. S. (2016). The Hevea brasiliensis XIP aquaporin subfamily : genomic, structural and functional characterizations with relevance to intensive latex harvesting. Plant Molecular Biology, 91 (4-5), p. 375-396. ISSN 0167-4412.

Titre du document
The Hevea brasiliensis XIP aquaporin subfamily : genomic, structural and functional characterizations with relevance to intensive latex harvesting
Année de publication
2016
Type de document
Article référencé dans le Web of Science WOS:000379037800001
Auteurs
Lopez D., Ben Amira M., Brown D., Muries B., Brunel-Michac N., Bourgerie S., Porcheron B., Lemoine R., Chrestin Hervé, Mollison E., Di Cola A., Frigerio L., Julien J. L., Gousset-Dupont A., Fumanal B., Label P., Pujade-Renaud V., Auguin D., Venisse J. S.
Source
Plant Molecular Biology, 2016, 91 (4-5), p. 375-396 ISSN 0167-4412
X-Intrinsic Proteins (XIP) were recently identified in a narrow range of plants as a full clade within the aquaporins. These channels reportedly facilitate the transport of a wide range of hydrophobic solutes. The functional roles of XIP in planta remain poorly identified. In this study, we found three XIP genes (HbXIP1;1, HbXIP2;1 and HbXIP3;1) in the Hevea brasiliensis genome. Comprehensive bioinformatics, biochemical and structural analyses were used to acquire a better understanding of this AQP subfamily. Phylogenetic analysis revealed that HbXIPs clustered into two major groups, each distributed in a specific lineage of the order Malpighiales. Tissue-specific expression profiles showed that only HbXIP2;1 was expressed in all the vegetative tissues tested (leaves, stem, bark, xylem and latex), suggesting that HbXIP2;1 could take part in a wide range of cellular processes. This is particularly relevant to the rubber-producing laticiferous system, where this isoform was found to be up-regulated during tapping and ethylene treatments. Furthermore, the XIP transcriptional pattern is significantly correlated to latex production level. Structural comparison with SoPIP2;1 from Spinacia oleracea species provides new insights into the possible role of structural checkpoints by which HbXIP2;1 ensures glycerol transfer across the membrane. From these results, we discuss the physiological involvement of glycerol and HbXIP2;1 in water homeostasis and carbon stream of challenged laticifers. The characterization of HbXIP2;1 during rubber tree tapping lends new insights into molecular and physiological response processes of laticifer metabolism in the context of latex exploitation.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020] ; Sciences du monde végétal [076]
Localisation
Fonds IRD [F B010067659]
Identifiant IRD
fdi:010067659
Contact