@article{fdi:010067625,
  title = {{S}imulation method linking dense microalgal culture spectral properties in the 400-750nm range to the physiology of the cells},
  author = {{B}ellini, {S}. and {B}endoula, {R}. and {L}e {F}loc'h, {E}. and {C}arr{\'e}, {C}laire and {M}as, {S}. and {V}idussi, {F}. and {F}ouilland, {E}. and {R}oger, {J}. {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}his work describes a method to model the optical properties over the (400-750nm) spectral range of a dense microalgal culture using the chemical and physical properties of the algal cells. {T}he method was based on a specific program called {A}lga{S}im coupled with the adding-doubling method: at the individual cell scale, {A}lga{S}im simulates the spectral properties of one model, three-layer spherical algal cell from its size and chemical composition. {A}s a second step, the adding-doubling method makes it possible to retrieve the total transmittance of the algal medium from the optical properties of the individual algal cells. {T}he method was tested by comparing the simulated total transmittance spectra for dense marine microalgal cultures of {I}sochrysis galbana (small flagellates) and {P}haeodactylum tricornutum (diatoms) to spectra measured using an experimental spectrophotometric setup. {O}ur study revealed that the total transmittance spectra simulated for the quasi-spherical cells of {I}sochrysis galbana were in good agreement with the measured spectra over the whole spectral range. {F}or {P}haeodactylum tricornutum, large differences between simulated and measured spectra were observed over the blue part of the transmittance spectra, probably due to non-spherical shape of the algal cells. {P}rediction of the algal cell density, mean size and pigment composition from the total transmittance spectra measured on algal samples was also investigated using the reversal of the method. {M}ean cell size was successfully predicted for both species. {T}he cell density was also successfully predicted for spherical {I}sochrysis galbana, with a relative error below 7%, but not for elongated {P}haeodactylum tricornutum with a relative error up to 26%. {T}he pigments total quantity and composition, the carotenoids:chlorophyll ratio in particular, were also successfully predicted for {I}sochrysis galbana with a relative error below 8%. {H}owever, the pigment predictions and measurements for {P}haeodactylum tricornutum showed large discrepancies, with a relative error up to 88%. {T}hese results give strong support for the development of a promising tool providing rapid and accurate estimations of biomass and physiological status of a dense microalgal culture based on only light transmittance properties.},
  keywords = {{A}dding-doubling ; dense algal culture monitoring ; multilayer model ; optical modeling of algal cells ; radiative transfer ; {M}ie theory},
  booktitle = {},
  journal = {{A}pplied {S}pectroscopy},
  volume = {70},
  numero = {6},
  pages = {1018--1033},
  ISSN = {0003-7028},
  year = {2016},
  DOI = {10.1177/0003702816641270},
  URL = {https://www.documentation.ird.fr/hor/fdi:010067625},
}