Publications des scientifiques de l'IRD

Nouwen Nico, Fardoux Joël, Giraud Eric. (2016). NodD1 and NodD2 are not required for the symbiotic interaction of Bradyrhizobium ORS285 with Nod-factor-independent Aeschynomene legumes. Plos One, 11 (6), p. e0157888 [12 p.]. ISSN 1932-6203.

Titre du document
NodD1 and NodD2 are not required for the symbiotic interaction of Bradyrhizobium ORS285 with Nod-factor-independent Aeschynomene legumes
Année de publication
2016
Type de document
Article référencé dans le Web of Science WOS:000378030000064
Auteurs
Nouwen Nico, Fardoux Joël, Giraud Eric
Source
Plos One, 2016, 11 (6), p. e0157888 [12 p.] ISSN 1932-6203
Photosynthetic Bradyrhizobium strain ORS285 forms nitrogen-fixing nodules on the roots and stems of tropical aquatic legumes of the Aeschynomene genus. Depending on the Aeschynomene species, this symbiotic interaction does or does not rely on the synthesis of Nod-factors (NFs). However, whether during the interaction of Bradyrhizobium ORS285 with NF-independent Aeschynomene species the nod genes are expressed and if the general regulator NodD plays a symbiotic role is unknown. Expression studies showed that in contrast to the interaction with the NF-dependent Aeschynomene species, A. afraspera, the Bradyrhizobium ORS285 nod genes are not induced upon contact with the NF-independent host plant A. indica. Mutational analysis of the two nodD genes present in ORS285, showed that deletion of nodD1 and nodD2 did not affect the symbiotic interaction between Bradyrhizobium ORS285 and A. indica whereas the deletions had an effect on the symbiotic interaction with A. afraspera plants. In addition, when the expression of nod genes was artificially induced by adding naringenin to the plant growth medium, the nodulation of A. indica by Bradyrhizobium ORS285 is delayed and resulted in lower nodule numbers.
Plan de classement
Biotechnologies [084]
Localisation
Fonds IRD [F B010067607]
Identifiant IRD
fdi:010067607
Contact