@article{fdi:010067317,
  title = {{S}hort-term liquid nitrogen storage of maize, common bean and soybean seeds sodifies their biochemical composition},
  author = {{A}rguedas, {M}. and {P}erez, {A}. and {A}bdelnour, {A}. and {H}ernandez, {M}. and {E}ngelmann, {F}lorent and {E}del {M}artínez, {M}. and {Y}abor, {L}. and {L}orenzo, {J}.{C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{W}e studied the effects of liquid nitrogen storage of maize, common bean and soybean seeds on their germination, electrolyte leakage, levels of chlorophylls, phenolics, aldehydes, proteins and peroxidase activity. {A}fter storage for 28 days, seeds were retrieved from liquid nitrogen, some were set to germinate and others were analyzed biochemically. {N}o phenotypic modifications were observed visually 5 days after beginning of germination, although percentage of seed germination was reduced by {LN} in maize and soybean. {M}oreover, numerous significant effects of seed cryopreservation were recorded at the biochemical status. {I}n maize seeds, the most important and statistically significant modifications were observed in the increased levels of chlorophyll b and total chlorophyll pigments and in the decreased contents of free phenolics after 28 days of exposure to {LN}, compared to the control treatment. {I}n common bean, relevant changes were observed in the increased electrolyte leakage and in the reduced levels of chlorophyll pigments (b, total) and free phenolics. {I}n soybean, modifications were observed in the increased levels of chlorophyll pigments (a, b, total), malondialdehyde and electrolyte leakage, and in the decreased peroxidase activity. {W}e have shown for the first time that immersion of maize, common bean and soybean seeds in liquid nitrogen modified the levels of different biochemicals.},
  keywords = {},
  booktitle = {},
  journal = {{A}gricultural {S}cience},
  volume = {4},
  numero = {3},
  pages = {6--12},
  ISSN = {2291-4471},
  year = {2016},
  URL = {https://www.documentation.ird.fr/hor/fdi:010067317},
}