@article{fdi:010067144,
  title = {{R}ecombinant forms of {L}eishmania amazonensis excreted/secreted promastigote surface antigen ({PSA}) induce protective immune responses in dogs},
  author = {{P}etitdidier, {E}lodie and {P}agniez, {J}ulie and {P}apierok, {G}. and {V}incendeau, {P}. and {L}emesre, {J}ean-{L}oup and {B}ras {G}oncalves, {R}achel},
  editor = {},
  language = {{ENG}},
  abstract = {{P}reventive vaccination is a highly promising strategy for interrupting leishmaniasis transmission that can, additionally, contribute to elimination. {A} vaccine formulation based on naturally excreted secreted ({ES}) antigens was prepared from {L}. infantum promastigote culture supernatant. {T}his vaccine achieved successful results in {P}hase {III} trials and was licensed and marketed as {C}ani{L}eish. {W}e recently showed that newly identified {ES} promastigote surface antigen ({PSA}), from both viable promastigotes and axenically-grown amastigotes, represented the major constituent and the highly immunogenic antigen of {L}. infantum and {L}. amazonensis {ES} products. {W}e report here that three immunizations with either the recombinant {ES} {L}a{PSA}-38{S} (r{PSA}) or its carboxy terminal part {L}a{PSA}-12{S} ({C}ter-r{PSA}), combined with {QA}-21 as adjuvant, confer high levels of protection in naive {L}. infantum-infected {B}eagle dogs, as checked by bone marrow parasite absence in respectively 78.8% and 80% of vaccinated dogs at 6 months post-challenge. {T}he parasite burden in infected vaccinated dogs was significantly reduced compared to placebo group, as measured by q-{PCR}. {M}oreover, our results reveal humoral and cellular immune response clear-cut differences between vaccinated and control dogs. {A}n early increase in specific {I}g{G}2 antibodies was observed in r{PSA}/{QA}-21- and {C}ter-r{PSA}/{QA}-21-immunized dogs only. {T}hey were found functionally active in vitro and were highly correlated with vaccine protection. {I}n vaccinated protected dogs, {IFN}-gamma and {NO} productions, as well as anti-leishmanial macrophage activity, were increased. {T}hese data strongly suggest that {ES} {PSA} or its carboxy-terminal part, in recombinant forms, induce protection in a canine model of zoonotic visceral leishmaniasis by inducing a {T}h1-dominant immune response and an appropriate specific antibody response. {T}hese data suggest that they could be considered as important active components in vaccine candidates.},
  keywords = {},
  booktitle = {},
  journal = {{PL}o{S} {N}eglected {T}ropical {D}iseases},
  volume = {10},
  numero = {5},
  pages = {art. e0004614 [18  en ligne]},
  ISSN = {1935-2735},
  year = {2016},
  DOI = {10.1371/journal.pntd.0004614},
  URL = {https://www.documentation.ird.fr/hor/fdi:010067144},
}