@article{fdi:010066982,
  title = {{DNA} barcoding and wing morphometrics to distinguish three {A}edes vectors in {T}hailand},
  author = {{S}umruayphol, {S}. and {A}piwathnasorn, {C}. and {R}uangsittichai, {J}. and {S}riwichai, {P}. and {A}ttrapadung, {S}. and {S}amung, {Y}. and {D}ujardin, {J}ean-{P}ierre},
  editor = {},
  language = {{ENG}},
  abstract = {{A}edes aegypti ({D}iptera: {C}ulicidae) ({L}.), {A}e. albopictus ({S}kuse), and {A}e. scutellaris ({W}alker) are important mosquito vectors of dengue and chikungunya viruses. {T}hey are morphologically similar and sympatric in some parts of their distribution; therefore, there is a risk of incorrect morphological identification. {A}ny confusion could have a negative impact on epidemiological studies or control strategies. {T}herefore, we explored two modern tools to supplement current morphological identification: {DNA} barcoding and geometric morphometric analyses. {F}ield larvae were reared to adults and carefully classified based on morphological traits. {T}he genetic analysis was based on the 658 bp each of 30{COI} sequences. {S}ome {C}ulex spp., {M}ansonia bonneae, were included as outgroups, and inclusion of a few other {A}edes spp. facilitated phylogenetic inference of the relationship between {A}e. albopictus and {A}e. scutellaris. {T}he two species were separated by an average interspecific divergence of 0.123 (0.119-0.127). {M}orphometric examination included landmark- (392 specimens) and outline-based (317 specimens) techniques. {T}he shape of the wing showed different discriminating power based on sex and digitizing technique. {T}his is the first time that {A}e. scutellaris and {A}e. albopictus have been compared using these two techniques. {W}e confirm that these morphologically close species are valid, and that geometric morphometrics can considerably increase the reliability of morphological identification.},
  keywords = {{A}edes scutellaris ; {A}e. aegypti ; {A}e. albopictus ; {L}andmarks ; {O}utlines ; {G}eometric morphometrics ; {THAILANDE}},
  booktitle = {},
  journal = {{A}cta {T}ropica},
  volume = {159},
  numero = {},
  pages = {1--10},
  ISSN = {0001-706{X}},
  year = {2016},
  DOI = {10.1016/j.actatropica.2016.03.010},
  URL = {https://www.documentation.ird.fr/hor/fdi:010066982},
}