@article{fdi:010066942,
  title = {{P}roteomic analysis of {P}lasmodium falciparum parasites from patients with cerebral and uncomplicated malaria},
  author = {{B}ertin, {G}wladys and {S}abbagh, {A}. and {A}rgy, {N}. and {S}alnot, {V}. and {E}zinmegnon, {S}. and {A}gbota, {G}. and {L}adipo, {Y}. and {A}lao, {J}. {M}. and {S}agbo, {G}. and {G}uillonneau, {F}. and {D}eloron, {P}hilippe},
  editor = {},
  language = {{ENG}},
  abstract = {{P}lasmodium falciparum is responsible of severe malaria, including cerebral malaria ({CM}). {D}uring its intra-erythrocytic maturation, parasite-derived proteins are expressed, exported and presented at the infected erythrocyte membrane. {T}o identify new {CM}-specific parasite membrane proteins, we conducted a mass spectrometry-based proteomic study and compared the protein expression profiles between 9 {CM} and 10 uncomplicated malaria ({UM}) samples. {A}mong the 1097 {P}lasmodium proteins identified, we focused on the 499 membrane-associated and hypothetical proteins for comparative analysis. {F}ilter-based feature selection methods combined with supervised data analysis identified a subset of 29 proteins distinguishing {CM} and {UM} samples with high classification accuracy. {A} hierarchical clustering analysis of these 29 proteins based on the similarity of their expression profiles revealed two clusters of 15 and 14 proteins, respectively under-and over-expressed in {CM}. {A}mong the over-expressed proteins, the {MESA} protein is expressed at the erythrocyte membrane, involved in proteins trafficking and in the export of variant surface antigens ({VSA}s), but without antigenic function. {A}ntigen 332 protein is exported at the erythrocyte, also involved in protein trafficking and in {VSA}s export, and exposed to the immune system. {O}ur proteomics data demonstrate an association of selected proteins in the pathophysiology of {CM}.},
  keywords = {{BENIN} ; {COTONOU}},
  booktitle = {},
  journal = {{S}cientific {R}eports - {N}ature},
  volume = {6},
  numero = {},
  pages = {art. 26773 [8 p.]},
  ISSN = {2045-2322},
  year = {2016},
  DOI = {10.1038/srep26773},
  URL = {https://www.documentation.ird.fr/hor/fdi:010066942},
}