@article{fdi:010066916,
  title = {{M}olecular species delimitation and morphology of aquatic and sub-aquatic bugs ({H}eteroptera) in {C}ameroon},
  author = {{E}bong, {S}. {M}. {A}. and {P}etit, {E}. and {L}e {G}all, {P}hilippe and {C}hen, {P}. {P}. and {N}ieser, {N}. and {G}uilbert, {E}. and {N}jiokou, {F}. and {M}arsollier, {L}. and {G}u{\'e}gan, {J}ean-{F}ran{\c{c}}ois and {P}luot-{S}igwalt, {D}. and {E}yangoh, {S}. and {H}arry, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A}quatic and semi-aquatic bugs ({H}eteroptera) represent a remarkable diversity and a resurging interest has been given to documenting at the species level these insects inhabiting {C}ameroon in {C}entral {A}frica due to their potential implication in the transmission of the bacterium {M}ycobacterium ulcerans, the causal agent of {B}uruli ulcer, an emerging human disease. {A} survey was carried out over two years in {C}ameroon. {M}orphological analyses were done in two steps. {A} first step consisted in separating the specimens based on broadly shared characters into morphotypes. {T}he specimens were then separated into two independent batches containing each the same representation of each morphotype. {O}ne batch (309 specimens) was used by taxonomy experts on aquatic bugs for species level identification and/or to reconcile nymph with their corresponding adult species. {T}he second batch (188 specimens) was used to define species based on the {COI} {DNA} sequences (standard sequence used for "{DNA} barcoding") and using the {A}utomatic {B}arcode {G}ap {D}iscovery ({ABGD}) method. {T}he first morphological analysis step separated the specimens into 63 different morphotypes (49 adults and 14 nymphs), which were then found to belong to 54 morphological species in the infra-orders {G}erromorpha and {N}epomorpha based on the species-level morphological identification, and 41-45 putative molecular species according to the gap value retained in the {ABGD}. {I}ntegrating morphology and "{DNA} barcoding" reconciled all the specimens into 62 aquatic bug species in {C}ameroon. {G}enerally, we obtained a good congruence between species a priori identified based on morphology from adult morphotypes and molecular putative species. {M}oreover, molecular identification has allowed the association of 86% of nymphs with adults. {T}his work illustrates the importance of integrative taxonomy.},
  keywords = {{CAMEROUN}},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {11},
  numero = {5},
  pages = {e0154905 [15 p.]},
  ISSN = {1932-6203},
  year = {2016},
  DOI = {10.1371/journal.pone.0154905},
  URL = {https://www.documentation.ird.fr/hor/fdi:010066916},
}