@article{fdi:010066904,
  title = {{G}lycans affect {DNA} extraction and induce substantial differences in gut metagenomic studies},
  author = {{A}ngelakis, {E}. and {B}achar, {D}. and {H}enrissat, {B}. and {A}rmougom, {F}abrice and {A}udoly, {G}. and {L}agier, {J}. {C}. and {R}obert, {C}. and {R}aoult, {D}idier},
  editor = {},
  language = {{ENG}},
  abstract = {{E}xopolysaccharides produced by bacterial species and present in feces are extremely inhibitory to {DNA} restriction and can cause discrepancies in metagenomic studies. {W}e determined the effects of different {DNA} extraction methods on the apparent composition of the gut microbiota using {I}llumina {M}i{S}eq deep sequencing technology. {DNA} was extracted from the stool from an obese female using 10 different methods and the choice of {DNA} extraction method affected the proportional abundance at the phylum level, species richness ({C}hao index, 227 to 2,714) and diversity (non parametric {S}hannon, 1.37 to 4.4). {M}oreover {DNA} was extracted from stools obtained from 83 different individuals by the fastest extraction assay and by an extraction assay that degradated exopolysaccharides. {T}he fastest extraction method was able to detect 68% to 100% genera and 42% to 95% species whereas the glycan degradation extraction method was able to detect 56% to 93% genera and 25% to 87% species. {T}o allow a good liberation of {DNA} from exopolysaccharides commonly presented in stools, we recommend the mechanical lysis of stools plus glycan degradation, used here for the first time. {C}aution must be taken in the interpretation of current metagenomic studies, as the efficiency of {DNA} extraction varies widely among stool samples.},
  keywords = {},
  booktitle = {},
  journal = {{S}cientific {R}eports - {N}ature},
  volume = {6},
  numero = {},
  pages = {art. 26276 [8 p.]},
  ISSN = {2045-2322},
  year = {2016},
  DOI = {10.1038/srep26276},
  URL = {https://www.documentation.ird.fr/hor/fdi:010066904},
}