@article{fdi:010066884,
  title = {{C}ellular and pectin dynamics during abscission zone development and ripe fruit abscission of the monocot oil palm},
  author = {{R}oongsattham, {P}. and {M}orcillo, {F}. and {F}ooyontphanich, {K}. and {J}antasuriyarat, {C}. and {T}ragoonrung, {S}. and {A}mblard, {P}. and {C}ollin, {M}yriam and {M}ouille, {G}. and {V}erdeil, {J}. {L}. and {T}ranbarger, {T}imothy},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he oil palm ({E}laeis guineensis {J}acq.) fruit primary abscission zone ({AZ}) is a multi-cell layered boundary region between the pedicel ({P}) and mesocarp ({M}) tissues. {T}o examine the cellular processes that occur during the development and function of the {AZ} cell layers, we employed multiple histological and immunohistochemical methods combined with confocal, electron and {F}ourier-transform infrared ({FT}-{IR}) microspectroscopy approaches. {D}uring early fruit development and differentiation of the {AZ}, the orientation of cell divisions in the {AZ} was periclinal compared with anticlinal divisions in the {P} and {M}. {AZ} cell wall width increased earlier during development suggesting cell wall assembly occurred more rapidly in the {AZ} than the adjacent {P} and {M} tissues. {T}he developing fruit {AZ} contain numerous intra-{AZ} cell layer plasmodesmata ({PD}), but very few inter-{AZ} cell layer {PD}. {I}n the {AZ} of ripening fruit, {PD} were less frequent, wider, and mainly intra-{AZ} cell layer localized. {F}urthermore, {DAPI} staining revealed nuclei are located adjacent to {PD} and are remarkably aligned within {AZ} layer cells, and remain aligned and intact after cell separation. {T}he polarized accumulation of ribosomes, rough endoplasmic reticulum, mitochondria, and vesicles suggested active secretion at the tip of {AZ} cells occurred during development which may contribute to the striated cell wall patterns in the {AZ} cell layers. {AZ} cells accumulated intracellular pectin during development, which appear to be released and/or degraded during cell separation. {T}he signal for the {JIM}5 epitope, that recognizes low methylesterified and un-methylesterified homogalacturonan ({HG}), increased in the {AZ} layer cell walls prior to separation and dramatically increased on the separated {AZ} cell surfaces. {F}inally, {FT}-{IR} microspectroscopy analysis indicated a decrease in methylesterified {HG} occurred in {AZ} cell walls during separation, which may partially explain an increase in the {JIM}5 epitope signal. {T}he results obtained through a multi-imaging approach allow an integrated view of the dynamic developmental processes that occur in a multi layered boundary {AZ} and provide evidence for distinct regulatory mechanisms that underlie oil palm fruit {AZ} development and function.},
  keywords = {fruit abscission ; abscission zone ; pectin ; oil palm ; cell wall},
  booktitle = {},
  journal = {{F}rontiers in {P}lant {S}cience},
  volume = {7},
  numero = {},
  pages = {540 [15 ]},
  ISSN = {1664-462{X}},
  year = {2016},
  DOI = {10.3389/fpls.2016.00540},
  URL = {https://www.documentation.ird.fr/hor/fdi:010066884},
}