@article{fdi:010066855, title = {{G}ene expression profiling of reproductive meristem types in early rice inflorescences by laser microdissection}, author = {{H}arrop, {T}. {W}. {R}. and {D}in, {I}. {U}. and {G}regis, {V}. and {O}snato, {M}. and {J}ouannic, {S}tefan and {A}dam, {H}{\'e}l{\`e}ne and {K}ater, {M}. {M}.}, editor = {}, language = {{ENG}}, abstract = {{I}n rice, inflorescence architecture is established at early stages of reproductive development and contributes directly to grain yield potential. {A}fter induction of flowering, the complexity of branching, and therefore the number of seeds on the panicle, is determined by the activity of different meristem types and the timing of transitions between them. {A}lthough some of the genes involved in these transitions have been identified, an understanding of the network of transcriptional regulators controlling this process is lacking. {T}o address this we used a precise laser microdissection and {RNA}-sequencing approach in {O}ryza sativa ssp. japonica cv. {N}ipponbare to produce quantitative data that describe the landscape of gene expression in four different meristem types: the rachis meristem, the primary branch meristem, the elongating primary branch meristem (including axillary meristems), and the spikelet meristem. {A} switch in expression profile between apical and axillary meristem types followed by more gradual changes during transitions in axillary meristem identity was observed, and several genes potentially involved in branching were identified. {T}his resource will be vital for a mechanistic understanding of the link between inflorescence development and grain yield.}, keywords = {inflorescence development ; rice ; reproductive meristems ; {RNA} sequencing}, booktitle = {}, journal = {{P}lant {J}ournal}, volume = {86}, numero = {1}, pages = {75--88}, ISSN = {0960-7412}, year = {2016}, DOI = {10.1111/tpj.13147}, URL = {https://www.documentation.ird.fr/hor/fdi:010066855}, }