%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Beck, I. A.
%A Payant, R.
%A Ngo-Giang-Huong, Nicole
%A Khamduang, W.
%A Laomanit, L.
%A Jourdain, Gonzague
%A Frenkel, L. M.
%T Development and validation of an oligonucleotide ligation assay to detect lamivudine resistance in hepatitis B virus
%D 2016
%L fdi:010066848
%G ENG
%J Journal of Virological Methods
%@ 0166-0934
%K HBV drug resistance ; Oligonucleotide ligation assay ; Lamivudine resistance ; HBV treatment
%K THAILANDE
%M ISI:000375506400009
%P 51-55
%R 10.1016/j.jviromet.2016.03.014
%U https://www.documentation.ird.fr/hor/fdi:010066848
%> https://www.documentation.ird.fr/intranet/publi/2016/05/010066848.pdf
%V 233
%W Horizon (IRD)
%X Treatment of chronic hepatitis B virus (HBV) infection with lamivudine-monotherapy rapidly selects mutant variants in a high proportion of individuals. Monitoring lamivudine resistance by consensus sequencing is costly and insensitive for detection of minority variants. An oligonucleotide ligation assay (OLA) for HBV lamivudine-resistance was developed and compared to consensus sequencing. Both assays detected drug resistance mutations in 35/64 (54.7%) specimens evaluated, and OLA detected minority mutants in an additional six (9.4%). OLA may offer a sensitive and inexpensive alternative to consensus sequencing for detection of HBV drug resistance in resource-limited settings.
%$ 052 ; 050