@article{fdi:010066848,
  title = {{D}evelopment and validation of an oligonucleotide ligation assay to detect lamivudine resistance in hepatitis {B} virus},
  author = {{B}eck, {I}. {A}. and {P}ayant, {R}. and {N}go-{G}iang-{H}uong, {N}icole and {K}hamduang, {W}. and {L}aomanit, {L}. and {J}ourdain, {G}onzague and {F}renkel, {L}. {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}reatment of chronic hepatitis {B} virus ({HBV}) infection with lamivudine-monotherapy rapidly selects mutant variants in a high proportion of individuals. {M}onitoring lamivudine resistance by consensus sequencing is costly and insensitive for detection of minority variants. {A}n oligonucleotide ligation assay ({OLA}) for {HBV} lamivudine-resistance was developed and compared to consensus sequencing. {B}oth assays detected drug resistance mutations in 35/64 (54.7%) specimens evaluated, and {OLA} detected minority mutants in an additional six (9.4%). {OLA} may offer a sensitive and inexpensive alternative to consensus sequencing for detection of {HBV} drug resistance in resource-limited settings.},
  keywords = {{HBV} drug resistance ; {O}ligonucleotide ligation assay ; {L}amivudine resistance ; {HBV} treatment ; {THAILANDE}},
  booktitle = {},
  journal = {{J}ournal of {V}irological {M}ethods},
  volume = {233},
  numero = {},
  pages = {51--55},
  ISSN = {0166-0934},
  year = {2016},
  DOI = {10.1016/j.jviromet.2016.03.014},
  URL = {https://www.documentation.ird.fr/hor/fdi:010066848},
}