Beck I. A., Payant R., Ngo-Giang-Huong Nicole, Khamduang W., Laomanit L., Jourdain Gonzague, Frenkel L. M. (2016). Development and validation of an oligonucleotide ligation assay to detect lamivudine resistance in hepatitis B virus. Journal of Virological Methods, 233, p. 51-55. ISSN 0166-0934.
Titre du document
Development and validation of an oligonucleotide ligation assay to detect lamivudine resistance in hepatitis B virus
Beck I. A., Payant R., Ngo-Giang-Huong Nicole, Khamduang W., Laomanit L., Jourdain Gonzague, Frenkel L. M.
Source
Journal of Virological Methods, 2016,
233, p. 51-55 ISSN 0166-0934
Treatment of chronic hepatitis B virus (HBV) infection with lamivudine-monotherapy rapidly selects mutant variants in a high proportion of individuals. Monitoring lamivudine resistance by consensus sequencing is costly and insensitive for detection of minority variants. An oligonucleotide ligation assay (OLA) for HBV lamivudine-resistance was developed and compared to consensus sequencing. Both assays detected drug resistance mutations in 35/64 (54.7%) specimens evaluated, and OLA detected minority mutants in an additional six (9.4%). OLA may offer a sensitive and inexpensive alternative to consensus sequencing for detection of HBV drug resistance in resource-limited settings.
