Ben Abdallah M., Karray F., Mhiri N., Mei N., Quéméneur Marianne, Cayol J. L., Erauso G., Tholozan Jean-Luc, Alazard Didier, Sayadi S. (2016). Prokaryotic diversity in a Tunisian hypersaline lake, Chott El Jerid. Extremophiles, 20 (2), p. 125-138. ISSN 1431-0651.
Titre du document
Prokaryotic diversity in a Tunisian hypersaline lake, Chott El Jerid
Année de publication
2016
Auteurs
Ben Abdallah M., Karray F., Mhiri N., Mei N., Quéméneur Marianne, Cayol J. L., Erauso G., Tholozan Jean-Luc, Alazard Didier, Sayadi S.
Source
Extremophiles, 2016,
20 (2), p. 125-138 ISSN 1431-0651
Prokaryotic diversity was investigated in a Tunisian salt lake, Chott El Jerid, by quantitative real-time PCR, denaturing gradient gel electrophoresis (DGGE) fingerprinting methods targeting the 16S rRNA gene and culture-dependent methods. Two different samples S1-10 and S2-10 were taken from under the salt crust of Chott El Jerid in the dry season. DGGE analysis revealed that bacterial sequences were related to Firmicutes, Proteobacteria, unclassified bacteria, and Deinococcus-Thermus phyla. Anaerobic fermentative and sulfate-reducing bacteria were also detected in this ecosystem. Within the domain archaea, all sequences were affiliated to Euryarchaeota phylum. Quantitative real-time PCR showed that 16S rRNA gene copy numbers of bacteria was 5 x 10(6) DNA copies g(-1) whereas archaea varied between 5 x 10(5) and 10(6) DNA copies g(-1) in these samples. Eight anaerobic halophilic fermentative bacterial strains were isolated and affiliated with the species Halanaerobium alcaliphilum, Halanaerobium saccharolyticum, and Sporohalobacter salinus. These data showed an abundant and diverse microbial community detected in the hypersaline thalassohaline environment of Chott El Jerid.
Plan de classement
Ecologie, systèmes aquatiques [036]
;
Biotechnologies [084]
Description Géographique
TUNISIE
Localisation
Fonds IRD [F B010066843]
Identifiant IRD
fdi:010066843
