@article{fdi:010066796,
  title = {{D}evelopment and mapping of functional expressed sequence tag-derived simple sequence repeat markers in a rubber tree {RRIM}600 x {PB}217 population},
  author = {{N}irapathpongporn, {K}. and {K}ongsawadworakul, {P}. and {V}iboonjun, {U}. and {T}eerawattanasuk, {K}. and {C}hrestin, {H}erv{\'e} and {S}egiun, {M}. and {C}lement-{D}ement, {A}. and {N}arangajavana, {J}.},
  editor = {},
  language = {{ENG}},
  abstract = {{S}ets of polymorphic expressed sequence tag-simple sequence repeat ({EST}-{SSR}) markers from the rubber tree ({H}evea brasiliensis) have been published by many researchers, but none has been specifically developed to study latex and wood yield traits. {I}n this study, a total 10,321 rubber tree {EST} sequences, generated from suppression subtractive hybridization-c{DNA} libraries of bark and latex of high-and low-yielding clones, were used as sources for {SSR} searching. {A} total of 432 {EST}-{SSR} loci were identified and it was possible to design primer pairs for a subset of 298 {EST}-{SSR}s. {T}he highest proportion of {EST}-{SSR}s was represented by dinucleotide repeats (46.6 %), followed by trinucleotide repeats (44.3 %). {B}ased on {BLASTX} analysis, 234 {EST}s (80 %) showed similarity to genes in {NCBI} databases and could be divided into 120 putative proteins with known function and 114 unknown proteins. {T}o enhance the resolution of an existing linkage map from previous work on a rubber tree {RRIM}600 x {PB}217 population, 69 {EST}-{SSR} markers from the above set were tested to be integrated into the reference genetic map. {T}he enriched map of 18 linkage groups spanned 2054.2 c{M} in length, showed an average genetic distance of 4.3 c{M} between adjacent markers, and included 63 new {EST}-{SSR} markers. {T}he enhanced map from this study provides a basis for comparative mapping using {PCR}-based markers and identification of expressed genes possibly affecting important traits of interest.},
  keywords = {{EST}-{SSR} marker ; {F}unctional markers ; {G}enetic linkage map ; {H}evea brasiliensis ; {R}ubber tree ; {THAILANDE}},
  booktitle = {},
  journal = {{M}olecular {B}reeding},
  volume = {36},
  numero = {4},
  pages = {art. 39 [9 p.]},
  ISSN = {1380-3743},
  year = {2016},
  DOI = {10.1007/s11032-016-0461-3},
  URL = {https://www.documentation.ird.fr/hor/fdi:010066796},
}