@article{fdi:010066784,
  title = {{PCR} of crt{NM} combined with analytical biochemistry : an efficient way to identify carotenoid producing lactic acid bacteria},
  author = {{T}urpin, {W}. and {R}enaud, {C}{\'e}cile and {A}vallone, {S}. and {H}ammoumi, {A}. and {G}uyot, {J}ean-{P}ierre and {H}umblot, {C}hrist{\`e}le},
  editor = {},
  language = {{ENG}},
  abstract = {{L}actic acid bacteria ({LAB}) synthesize a wide variety of biochemical compounds during food fermentation. {C}arotenoids provide important biological functions for bacteria, and their consumption by humans has many beneficial effects. {I}n this study, the presence of several genes involved in the production of carotenoids was determined by {BLAST} analysis and {PCR} in a collection of 156 {LAB} isolated from traditional amylaceous {A}frican fermented foods. {O}nly the crt{E} gene and the crt{NM} operon were present and detected in {L}actobacillaceae. {M}ost of the strains with positive {PCR} detection of the operon crt{NM} produced carotenoid-like compounds when grown in {MRS} broth. {T}he carotenoids produced differed from compounds previously identified in other {LAB} except for one peak, which was closely related to 4,4'-diaponeurosporene already reported in the literature in {L}actobacillus plantarum species. {M}ost producing strains belonged to {L}actobacillus fermentum and {L}. plantarum species but a few {P}ediococcus acidilactici were also producers. {F}urthermore, the most efficient {L}. plantarum was able to synthesize carotenoids in a cereal fermented food. {G}enetic screening was shown to be efficient since, in all cases, it eliminated the need for biochemical analysis of strains in which no amplicons of the operon crt{NM} were obtained.},
  keywords = {{C}arotenoid ; {F}ood fermentation ; {G}enetic ; {L}actobacillus ; {P}ediococcus ; {V}itamin {A} ; {BURKINA} {FASO}},
  booktitle = {},
  journal = {{S}ystematic and {A}pplied {M}icrobiology},
  volume = {39},
  numero = {2},
  pages = {115--121},
  ISSN = {0723-2020},
  year = {2016},
  DOI = {10.1016/j.syapm.2015.12.003},
  URL = {https://www.documentation.ird.fr/hor/fdi:010066784},
}