@article{fdi:010066745,
  title = {{A} molecular method to discriminate between mass-reared sterile and wild tsetse flies during eradication programmes that have a sterile insect technique component},
  author = {{P}agabeleguem, {S}. and {G}imonneau, {G}. and {S}eck, {M}. {T}. and {V}reysen, {M}. {J}. {B}. and {S}all, {B}. and {R}ayaiss{\'e}, {J}. {B}. and {S}idib{\'e}, {I}. and {B}ouyer, {J}. and {R}avel, {S}ophie},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground {T}he {G}overnment of {S}enegal has embarked several years ago on a project that aims to eradicate {G}lossina palpalis gambiensis from the {N}iayes area. {T}he removal of the animal try-panosomosis would allow the development more efficient livestock production systems. {T}he project was implemented using an area-wide integrated pest management strategy including a sterile insect technique ({SIT}) component. {T}he released sterile male flies originated from a colony from {B}urkina {F}aso. {M}ethodology/{P}rincipal {F}indings {M}onitoring the efficacy of the sterile male releases requires the discrimination between wild and sterile male {G}.p. gambiensis that are sampled in monitoring traps. {B}efore being released, sterile male flies were marked with a fluorescent dye powder. {T}he marking was however not infallible with some sterile flies only slightly marked or some wild flies contaminated with a few dye particles in the monitoring traps. {T}rapped flies can also be damaged due to predation by ants, making it difficult to discriminate between wild and sterile males using a fluorescence camera and / or a fluorescence microscope. {W}e developed a molecular technique based on the determination of cytochrome oxidase haplotypes of {G}. p. gambiensis to discriminate between wild and sterile males. {DNA} was isolated from the head of flies and a portion of the 5' end of the mitochondrial gene cytochrome oxidase {I} was amplified to be finally sequenced. {O}ur results indicated that all the sterile males from the {B}urkina {F}aso colony displayed the same haplotype and systematically differed from wild male flies trapped in {S}enegal and {B}urkina {F}aso. {T}his allowed 100% discrimination between sterile and wild male {G}. p. gambiensis. {C}onclusions/{S}ignificance {T}his tool might be useful for other tsetse control campaigns with a {SIT} component in the framework of the {P}an-{A}frican {T}setse and {T}rypanosomosis {E}radication {C}ampaign ({PATTEC}) and, more generally, for other vector or insect pest control programs.},
  keywords = {{SENEGAL} ; {BURKINA} {FASO}},
  booktitle = {},
  journal = {{P}los {N}eglected {T}ropical {D}iseases},
  volume = {10},
  numero = {2},
  pages = {e0004491 [11 p.]},
  ISSN = {1935-2735},
  year = {2016},
  DOI = {10.1371/journal.pntd.0004491},
  URL = {https://www.documentation.ird.fr/hor/fdi:010066745},
}