@article{fdi:010066674,
  title = {{I}dentification of a {T}sal1(52-75) salivary synthetic peptide to monitor cattle exposure to tsetse flies},
  author = {{S}omda, {M}. {B}. and {C}orn{\'e}lie, {S}ylvie and {B}engaly, {Z}. and {M}athieu-{D}aud{\'e}, {F}ran{\c{c}}oise and {P}oinsignon, {A}nne and {D}ama, {E}. and {B}ouyer, {J}. and {S}idibe, {I}. and {D}emettre, {E}. and {S}eveno, {M}. and {R}emou{\'e}, {F}ranck and {S}anon, {A}. and {B}ucheton, {B}runo},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {T}he saliva of tsetse flies contains a cocktail of bioactive molecules inducing specific antibody responses in hosts exposed to bites. {W}e have previously shown that an indirect-{ELISA} test using whole salivary extracts from {G}lossina morsitans submorsitans was able to discriminate between (i) cattle from tsetse infested and tsetse free areas and (ii) animals experimentally exposed to low or high numbers of tsetse flies. {I}n the present study, our aim was to identify specific salivary synthetic peptides that could be used to develop simple immunoassays to measure cattle exposure to tsetse flies. {M}ethods: {I}n a first step, 2{D}-electrophoresis immunoblotting, using sera from animals exposed to a variety of bloodsucking arthropods, was performed to identify specific salivary proteins recognised in cattle exposed to tsetse bites. {L}inear epitope prediction software and {B}last analysis were then used to design synthetic peptides within the identified salivary proteins. {F}inally, candidate peptides were tested by indirect-{ELISA} on serum samples from tsetse infested and tsetse free areas, and from exposure experiments. {R}esults: {T}he combined immunoblotting and bioinformatics analyses led to the identification of five peptides carrying putative linear epitopes within two salivary proteins: the tsetse salivary gland protein 1 ({T}sal1) and the {S}alivary {S}ecreted {A}denosine ({SSA}). {O}f these, two were synthesised and tested further based on the absence of sequence homology with other arthropods or pathogen species. {I}g{G} responses to the {T}sal1(52-75) synthetic peptide were shown to be specific of tsetse exposure in both naturally and experimentally exposed hosts. {N}evertheless, anti-{T}sal1(52-75) {I}g{G} responses were absent in animals exposed to high tsetse biting rates. {C}onclusions: {T}hese results suggest that {T}sal1(52-75) specific antibodies represent a biomarker of low cattle exposure to tsetse fly. {T}hese results are discussed in the light of the other available tsetse saliva based-immunoassays and in the perspective of developing a simple serological tool for tsetse eradication campaigns to assess the tsetse free status or to detect tsetse reemergence in previously cleared areas.},
  keywords = {{S}ynthetic {P}eptide ; {B}iomarker of {E}xposure ; {C}attle ; {T}setse {F}lies ; {A}frican {A}nimal {T}rypanosomosis ; {BURKINA} {FASO}},
  booktitle = {},
  journal = {{P}arasites and {V}ectors},
  volume = {9},
  numero = {},
  pages = {art. 149 [12 p.]},
  ISSN = {1756-3305},
  year = {2016},
  DOI = {10.1186/s13071-016-1414-8},
  URL = {https://www.documentation.ird.fr/hor/fdi:010066674},
}