@article{fdi:010066198, title = {{D}evelopment and characterization of polymorphic microsatellite markers in neotropical fish of the genus {A}pistogramma ({P}erciformes : {L}abroidei : {C}ichlidae)}, author = {{Q}u{\'e}rouil, {S}ophie and {D}iaz, {A}. {V}. and {G}arcia-{D}avila, {C}. and {R}omer, {U}. and {R}enno, {J}ean-{F}ran{\c{c}}ois}, editor = {}, language = {{ENG}}, abstract = {{T}he {S}outh {A}merican genus of {C}ichlid fish {A}pistogramma comprises over 100 species, most of which are difficult to identify. {T}here is a need to clarify species limits and evolutionary relationships, conduct fine-scale phylogeographic revision of some species complexes, and collect information on population conservation status. {I}n addition, recent studies suggest that female mate choice may lead to reproductive isolation and potentially to sympatric speciation in some species. {H}ighly variable bi-parentally inherited molecular markers are needed to determine reproductive units in order to test this hypothesis. {M}icrosatellite markers were developed for fine-scale population genetic analyses within the genus {A}pistogramma, based on a {DNA} library of 7567 candidate loci. {A} set of 40 loci with dinucleotide repeats was selected and tested for amplification and dimorphism in three {A}pistogramma species (six samples). {T}hirteen polymorphic loci, combined in three multiplex sets, were retained and analyzed in nine {A}pistogramma species (47 samples) representative of the genus diversity in the {P}eruvian {A}mazon basin. {A}ll loci presented a high level of heterozygosity and polymorphism in most species. {T}his will be helpful in evolutionary genetics and conservation studies.}, keywords = {{PEROU} ; {AMAZONIE} ; {AMAZONE} {BASSIN}}, booktitle = {{P}roceedings of the 4th {I}nternational {C}onference of the "{R}esearch {N}etwork on {A}mazonian {I}chthyofauna" ({RIIA})}, journal = {{J}ournal of {A}pplied {I}chthyology}, volume = {31}, numero = {{S}4}, pages = {52--56}, ISSN = {0175-8659}, year = {2015}, DOI = {10.1111/jai.12975}, URL = {https://www.documentation.ird.fr/hor/fdi:010066198}, }