Publications des scientifiques de l'IRD

Lecarpentier E., Bhatt M., Bertin G. I., Deloison B., Salomon L. J., Deloron Philippe, Fournier T., Barakat A. I., Tsatsaris V. (2016). Computational fluid dynamic simulations of maternal circulation : wall shear stress in the human placenta and its biological implications. Plos One, 11 (1), p. e0147262 [ 18 p.]. ISSN 1932-6203.

Titre du document
Computational fluid dynamic simulations of maternal circulation : wall shear stress in the human placenta and its biological implications
Année de publication
2016
Type de document
Article référencé dans le Web of Science WOS:000369528200028
Auteurs
Lecarpentier E., Bhatt M., Bertin G. I., Deloison B., Salomon L. J., Deloron Philippe, Fournier T., Barakat A. I., Tsatsaris V.
Source
Plos One, 2016, 11 (1), p. e0147262 [ 18 p.] ISSN 1932-6203
Introduction In the human placenta the maternal blood circulates in the intervillous space (IVS). The syncytiotrophoblast (STB) is in direct contact with maternal blood. The wall shear stress (WSS) exerted by the maternal blood flow on the STB has not been evaluated. Our objective was to determine the physiological WSS exerted on the surface of the STB during the third trimester of pregnancy. Material and Methods To gain insight into the shear stress levels that the STB is expected to experience in vivo, we have formulated three different computational models of varying levels of complexity that reflect different physical representations of the IVS. Computations of the flow fields in all models were performed using the CFD module of the finite element code COMSOL Multi-physics 4.4. The mean velocity of maternal blood in the IVS during the third trimester was measured in vivo with dynamic MRI (0.94 +/- 0.14 mm.s(-1)). To investigate if the in silico results are consistent with physiological observations, we studied the cytoadhesion of human parasitized (Plasmodium falciparum) erythrocytes to primary human STB cultures, in flow conditions with different WSS values. Results The WSS applied to the STB is highly heterogeneous in the IVS. The estimated average values are relatively low (0.5 +/- 0.2 to 2.3 +/- 1.1 dyn.cm(-2)). The increase of WSS from 0.15 to 5 dyn.cm(-2) was associated with a significant decrease of infected erythrocyte cytoadhesion. No cytoadhesion of infected erythrocytes was observed above 5 dyn.cm(-2) applied for one hour. Conclusion Our study provides for the first time a WSS estimation in the maternal placental circulation. In spite of high maternal blood flow rates, the average WSS applied at the surface of the chorionic villi is low (<5 dyn.cm(-2)). These results provide the basis for future physiologicallyrelevant in vitro studies of the biological effects of WSS on the STB.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020] ; Santé : généralités [050] ; Entomologie médicale / Parasitologie / Virologie [052]
Localisation
Fonds IRD [F B010066191]
Identifiant IRD
fdi:010066191
Contact