@article{fdi:010066114,
  title = {{A} knowledge-based molecular screen uncovers a broad-spectrum {O}s{SWEET}14 resistance allele to bacterial blight from wild rice},
  author = {{H}utin, {M}athilde and {S}abot, {F}ran{\c{c}}ois and {G}hesqui{\`e}re, {A}lain and {K}oebnik, {R}alf and {S}zurek, {B}oris},
  editor = {},
  language = {{ENG}},
  abstract = {{T}ranscription activator-like ({TAL}) effectors are type {III}-delivered transcription factors that enhance the virulence of plant pathogenic {X}anthomonas species through the activation of host susceptibility ({S}) genes. {TAL} effectors recognize their {DNA} target(s) via a partially degenerate code, whereby modular repeats in the {TAL} effector bind to nucleotide sequences in the host promoter. {A}lthough this knowledge has greatly facilitated our power to identify new {S} genes, it can also be easily used to screen plant genomes for variations in {TAL} effector target sequences and to predict for loss-of-function gene candidates in silico. {I}n a proof-of-principle experiment, we screened a germplasm of 169 rice accessions for polymorphism in the promoter of the major bacterial blight susceptibility {S} gene {O}s{SWEET}14, which encodes a sugar transporter targeted by numerous strains of {X}anthomonas oryzae pv. oryzae. {W}e identified a single allele with a deletion of 18 bp overlapping with the binding sites targeted by several {TAL} effectors known to activate the gene. {W}e show that this allele, which we call xa41(t), confers resistance against half of the tested {X}oo strains, representative of various geographic origins and genetic lineages, highlighting the selective pressure on the pathogen to accommodate {O}s{SWEET}14 polymorphism, and reciprocally the apparent limited possibilities for the host to create variability at this particular {S} gene. {A}nalysis of xa41(t) conservation across the {O}ryza genus enabled us to hypothesize scenarios as to its evolutionary history, prior to and during domestication. {O}ur findings demonstrate that resistance through {TAL} effector-dependent loss of {S}-gene expression can be greatly fostered upon knowledge-based molecular screening of a large collection of host plants.},
  keywords = {{O}ryza barthii ; susceptibility ({S}) gene ; {X}anthomonas oryzae ; {TAL} effector ; {SWEET} ; xa41(t)},
  booktitle = {},
  journal = {{P}lant {J}ournal},
  volume = {84},
  numero = {4},
  pages = {694--703},
  ISSN = {0960-7412},
  year = {2015},
  DOI = {10.1111/tpj.13042},
  URL = {https://www.documentation.ird.fr/hor/fdi:010066114},
}