@article{fdi:010066076,
  title = {{M}onoclonal antibodies for the diagnosis of {B}orrelia crocidurae},
  author = {{F}otso, {A}. {F}. and {M}ediannikov, {O}leg and {N}appez, {C}. and {A}zza, {S}. and {R}aoult, {D}idier and {D}rancourt, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{R}elapsing fever borreliae, produced by ectoparasite-borne {B}orrelia species, cause mild to deadly bacteremia and miscarriage. {I}n the perspective of developing inexpensive assays for the rapid detection of relapsing fever borreliae, we produced 12 monoclonal antibodies ({MA}bs) against {B}orrelia crocidurae and characterized the two exhibiting the highest titers. {P}3{A}10 {MA}b reacts with the 35.6-k{D}a flagellin {B} (fla{B}) of {B}. crocidurae while {P}6{D}9 {MA}b recognizes a 35.1-k{D}a variable-like protein ({V}lp) in {B}. crocidurae and a 35.2-k{D}a {V}lp in {B}orrelia duttonii. {I}ndirect immunofluorescence assay incorporating relapsing fever and {L}yme group borreliae and 11 blood-borne organisms responsible for fever in {W}est {A}frica confirmed the reactivity of these two {MA}bs. {C}ombining these two {MA}bs in indirect immunofluorescence assays detected relapsing fever borreliae including {B}. crocidurae in ticks and the blood of febrile {S}enegalese patients. {B}oth antibodies could be incorporated into inexpensive and stable formats suited for the rapid point-of-care diagnosis of relapsing fever. {T}hese first-ever {MA}bs directed against {A}frican relapsing fever borreliae are available for the scientific community to promote research in this neglected field.},
  keywords = {{AFRIQUE} {DE} {L}'{OUEST} ; {SENEGAL}},
  booktitle = {},
  journal = {{A}merican {J}ournal of {T}ropical {M}edicine and {H}ygiene},
  volume = {94},
  numero = {1},
  pages = {61--67},
  ISSN = {0002-9637},
  year = {2016},
  DOI = {10.4269/ajtmh.15-0436},
  URL = {https://www.documentation.ird.fr/hor/fdi:010066076},
}