Publications des scientifiques de l'IRD

van Bruggen R., Gualtieri C., Iliescu A., Cheepsunthorn C. L., Mungkalasut P., Trape Jean-François, Modiano D., Sirima B. S., Singhasivanon P., Lathrop M., Sakuntabhai A., Bureau J. F., Gros P. (2015). Modulation of malaria phenotypes by pyruvate kinase (PKLR) variants in a Thai population. Plos One, 10 (12), p. e0144555 [18 p.]. ISSN 1932-6203.

Titre du document
Modulation of malaria phenotypes by pyruvate kinase (PKLR) variants in a Thai population
Année de publication
2015
Type de document
Article référencé dans le Web of Science WOS:000366715900062
Auteurs
van Bruggen R., Gualtieri C., Iliescu A., Cheepsunthorn C. L., Mungkalasut P., Trape Jean-François, Modiano D., Sirima B. S., Singhasivanon P., Lathrop M., Sakuntabhai A., Bureau J. F., Gros P.
Source
Plos One, 2015, 10 (12), p. e0144555 [18 p.] ISSN 1932-6203
Pyruvate kinase (PKLR) is a critical erythrocyte enzyme that is required for glycolysis and production of ATP. We have shown that Pklr deficiency in mice reduces the severity (reduced parasitemia, increased survival) of blood stage malaria induced by infection with Plasmodium chabaudi AS. Likewise, studies in human erythrocytes infected ex vivo with P. falciparum show that presence of host PK-deficiency alleles reduces infection phenotypes. We have characterized the genetic diversity of the PKLR gene, including haplotype structure and presence of rare coding variants in two populations from malaria endemic areas of Thailand and Senegal. We investigated the effect of PKLR genotypes on rich longitudinal datasets including haematological and malaria-associated phenotypes. A coding and possibly damaging variant (R41Q) was identified in the Thai population with a minor allele frequency of similar to 4.7%. Arginine 41 (R41) is highly conserved in the pyruvate kinase family and its substitution to Glutamine (R41Q) affects protein stability. Heterozygosity for R41Q is shown to be associated with a significant reduction in the number of attacks with Plasmodium falciparum, while correlating with an increased number of Plasmodium vivax infections. These results strongly suggest that PKLR protein variants may affect the frequency, and the intensity of malaria episodes induced by different Plasmodium parasites in humans living in areas of endemic malaria.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020] ; Entomologie médicale / Parasitologie / Virologie [052]
Description Géographique
THAILANDE
Localisation
Fonds IRD [F B010066047]
Identifiant IRD
fdi:010066047
Contact