@article{fdi:010066039,
  title = {{C}hitinase-resistant hydrophilic symbiotic factors secreted by {F}rankia activate both {C}a2+ spiking and {NIN} gene expression in the actinorhizal plant {C}asuarina glauca},
  author = {{C}habaud, {M}. and {G}herbi, {H}assen and {P}irolles, {E}lodie and {V}aissayre, {V}irginie and {F}ournier, {J}. and {M}oukouanga, {D}aniel and {F}ranche, {C}laudine and {B}ogusz, {D}idier and {T}isa, {L}. {S}. and {B}arker, {D}. {G}. and {S}vistoonoff, {S}ergio},
  editor = {},
  language = {{ENG}},
  abstract = {{A}lthough it is now well-established that decorated lipo-chitooligosaccharide {N}od factors are the key rhizobial signals which initiate infection/nodulation in host legume species, the identity of the equivalent microbial signaling molecules in the {F}rankia/actinorhizal association remains elusive. {W}ith the objective of identifying {F}rankia symbiotic factors we present a novel approach based on both molecular and cellular pre-infection reporters expressed in the model actinorhizal species {C}asuarina glauca. {B}y introducing the nuclear-localized cameleon {N}up-{YC}2.1 into {C}asuarina glauca we show that cell-free culture supernatants of the compatible {F}rankia {C}c{I}3 strain are able to elicit sustained high frequency {C}a2+ spiking in host root hairs. {F}urthermore, an excellent correlation exists between the triggering of nuclear {C}a2+ spiking and the transcriptional activation of the {P}ro{C}g{NIN}:{GFP} reporter as a function of the {F}rankia strain tested. {T}hese two pre-infection symbiotic responses have been used in combination to show that the signal molecules present in the {F}rankia {C}c{I}3 supernatant are hydrophilic, of low molecular weight and resistant to chitinase degradation. {I}n conclusion, the biologically active symbiotic signals secreted by {F}rankia appear to be chemically distinct from the currently known chitin-based rhizobial/arbuscular mycorrhizal signaling molecules. {C}onvenient bioassays in {C}asuarina glauca are now available for their full characterization.},
  keywords = {actinorhiza ; chitinase-resistance ; {F}rankia culture supernatant ; {NIN} gene expression ; nuclear {C}a2+ spiking ; plant-microbe signaling ; transgenic ; {C}asuarina glauca},
  booktitle = {},
  journal = {{N}ew {P}hytologist},
  volume = {209},
  numero = {1},
  pages = {86--93},
  ISSN = {0028-646{X}},
  year = {2016},
  DOI = {10.1111/nph.13732},
  URL = {https://www.documentation.ird.fr/hor/fdi:010066039},
}