@article{fdi:010066022,
  title = {{S}eed-specific stable expression of the alpha-{AI}1 inhibitor in coffee grains and the in vivo implications for the development of the coffee berry borer},
  author = {{A}lbuquerque, {E}. {V}. {S}. and {B}ezerra, {C}. {A}. and {R}omero, {J}. {V}. and {V}alencia, {J}. {W}. {A}. and {V}alencia-{J}imenez, {A}. and {P}imenta, {L}. {M}. and {B}arbosa, {A}ead and {S}ilva, {M}. {C}. {M}. and {M}eneguim, {A}. {M}. and {S}a, {M}. {E}. {L}. and {E}ngler, {G}. and de {A}lmeida-{E}ngler, {J}. and {F}ernandez, {D}iana and {G}rossi-de-{S}a, {M}. {F}.},
  editor = {},
  language = {{ENG}},
  abstract = {{G}enetic transformation of coffee ({C}offea spp.), the second most traded commodity worldwide, is an alternative approach to introducing features that cannot be introgressed by traditional crossings. {T}he transgenic stability, heritability and quantitative and spatial expression patterns of the seed-specific promoter phytohemagglutinin ({PHA}-{L}) from {P}haseolus vulgaris were characterized in genetically modified {C}. arabica expressing the alpha-amylase inhibitor-1 (alpha-{AI}1) gene. {T}he alpha-{AI}1 inhibitor shows considerable activity toward digestive enzymes of the coffee berry borer ({CBB}) {H}ypothenemus hampei. {T}his insect pest expends its life cycle almost entirely in coffee berries. {T}ransgene containment in the fruit is important to meeting food and environmental safety requirements for releasing genetically modified ({GM}) crops. {PCR} analysis of {T}2 coffee plants showed a {M}endelian single-copy segregation pattern. {E}ctopic transgene expression was only detected in coffee grains, as demonstrated by reverse transcription-{PCR} analysis of different plant tissues. {A}n intense immunocytochemical signal associated with alpha-{AI}1 protein expression was localized to endospermic cells. {I}n addition, a delay in the larval development of {CBB} was observed after challenging transgenic coffee seeds with the insect. {T}hese results indicate that the {PHA}-{L} promoter might be a useful tool in coffee for the seed-specific expression of genes related to coffee bean productivity, quality and pest protection. {T}he biotechnological applicability of the alpha-{AI}1 gene for controlling {CBB} is also discussed. {T}his work is the first report showing a seed-specific transgene expression in coffee plants.},
  keywords = {{F}ruit-specific expression ; {H}ypothenemus hampei ; {G}enetically modified plants ; {C}offea arabica ; {I}mmunolocalization},
  booktitle = {},
  journal = {{T}ropical {P}lant {B}iology},
  volume = {8},
  numero = {3-4},
  pages = {98--107},
  ISSN = {1935-9756},
  year = {2015},
  DOI = {10.1007/s12042-015-9153-0},
  URL = {https://www.documentation.ird.fr/hor/fdi:010066022},
}