Publications des scientifiques de l'IRD

Albuquerque E. V. S., Bezerra C. A., Romero J. V., Valencia J. W. A., Valencia-Jimenez A., Pimenta L. M., Barbosa Aead, Silva M. C. M., Meneguim A. M., Sa M. E. L., Engler G., de Almeida-Engler J., Fernandez Diana, Grossi-de-Sa M. F. (2015). Seed-specific stable expression of the alpha-AI1 inhibitor in coffee grains and the in vivo implications for the development of the coffee berry borer. Tropical Plant Biology, 8 (3-4), p. 98-107. ISSN 1935-9756.

Titre du document
Seed-specific stable expression of the alpha-AI1 inhibitor in coffee grains and the in vivo implications for the development of the coffee berry borer
Année de publication
2015
Type de document
Article référencé dans le Web of Science WOS:000366372400005
Auteurs
Albuquerque E. V. S., Bezerra C. A., Romero J. V., Valencia J. W. A., Valencia-Jimenez A., Pimenta L. M., Barbosa Aead, Silva M. C. M., Meneguim A. M., Sa M. E. L., Engler G., de Almeida-Engler J., Fernandez Diana, Grossi-de-Sa M. F.
Source
Tropical Plant Biology, 2015, 8 (3-4), p. 98-107 ISSN 1935-9756
Genetic transformation of coffee (Coffea spp.), the second most traded commodity worldwide, is an alternative approach to introducing features that cannot be introgressed by traditional crossings. The transgenic stability, heritability and quantitative and spatial expression patterns of the seed-specific promoter phytohemagglutinin (PHA-L) from Phaseolus vulgaris were characterized in genetically modified C. arabica expressing the alpha-amylase inhibitor-1 (alpha-AI1) gene. The alpha-AI1 inhibitor shows considerable activity toward digestive enzymes of the coffee berry borer (CBB) Hypothenemus hampei. This insect pest expends its life cycle almost entirely in coffee berries. Transgene containment in the fruit is important to meeting food and environmental safety requirements for releasing genetically modified (GM) crops. PCR analysis of T2 coffee plants showed a Mendelian single-copy segregation pattern. Ectopic transgene expression was only detected in coffee grains, as demonstrated by reverse transcription-PCR analysis of different plant tissues. An intense immunocytochemical signal associated with alpha-AI1 protein expression was localized to endospermic cells. In addition, a delay in the larval development of CBB was observed after challenging transgenic coffee seeds with the insect. These results indicate that the PHA-L promoter might be a useful tool in coffee for the seed-specific expression of genes related to coffee bean productivity, quality and pest protection. The biotechnological applicability of the alpha-AI1 gene for controlling CBB is also discussed. This work is the first report showing a seed-specific transgene expression in coffee plants.
Plan de classement
Sciences du monde végétal [076]
Localisation
Fonds IRD [F B010066022]
Identifiant IRD
fdi:010066022
Contact