HLA-E coding and 3 ' untranslated region variability determined by next-generation sequencing in two West-African population samples

2015

Article référencé dans le Web of Science WOS:000366437900011

Castelli E. C., Mendes C. T., Sabbagh A., Porto I. O. P., Garcia André, Ramalho J., Lima T. H. A., Massaro J. D., Dias F. C., Collares C. V. A., Jamonneau Vincent, Bucheton Bruno, Camara M., Donadi E. A.

Human Immunology, 2015, 76 (12), p. 945-953 ISSN 0198-8859

HLA-E is a non-classical Human Leucocyte Antigen class I gene with immunomodulatory properties. Whereas HLA-E expression usually occurs at low levels, it is widely distributed amongst human tissues, has the ability to bind self and non-self antigens and to interact with NK cells and T lymphocytes, being important for immunosurveillance and also for fighting against infections. HLA-E is usually the most conserved locus among all class I genes. However, most of the previous studies evaluating HLA-E variability sequenced only a few exons or genotyped known polymorphisms. Here we report a strategy to evaluate HLA-E variability by next-generation sequencing (NGS) that might be used to other HLA loci and present the HLA-E haplotype diversity considering the segment encoding the entire HLA-E mRNA (including 5'UTR, introns and the 3'UTR) in two African population samples, Susu from Guinea-Conakry and Lobi from Burkina Faso. Our results indicate that (a) the HLA-E gene is indeed conserved, encoding mainly two different protein molecules; (b) Africans do present several unknown HLA-E alleles presenting synonymous mutations; (c) the HLA-E 3'UTR is quite polymorphic and (d) haplotypes in the HLA-E 3'UTR are in close association with HLA-E coding alleles. NGS has proved to be an important tool on data generation for future studies evaluating variability in non-classical MHC genes.

Santé : généralités [050] ; Société, développement social [106]

GUINEE ; BURKINA FASO

Fonds IRD [F B010066010]

fdi:010066010