%0 Journal Article %9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES %A Fadhlaoui, K. %A Ben Hania, Wajdi %A Postec, A. %A Fauque, G. %A Hamdi, M. %A Ollivier, Bernard %A Fardeau, Marie-Laure %T Fusibacter fontis sp. nov., a sulfur-reducing, anaerobic bacterium isolated from a mesothermic Tunisian spring %D 2015 %L fdi:010065515 %G ENG %J International Journal of Systematic and Evolutionary Microbiology %@ 1466-5026 %K TUNISIE ; BIZERTE %M ISI:000366152100042 %N 10 %P 3501-3506 %R 10.1099/ijsem.0.000445 %U https://www.documentation.ird.fr/hor/fdi:010065515 %> https://www.documentation.ird.fr/intranet/publi/2015/12/010065515.pdf %V 65 %W Horizon (IRD) %X Strain KhalAKB1(T), a mesophilic, anaerobic, rod-shaped bacterium, was isolated from water collected from a mesothermic Tunisian spring. Cells were Gram-staining-Positive rods, occurring singly or in pairs and motile by one lateral flagellum. Strain KhalAKB1(T) grew at 15-45 degrees C (optimum 30 degrees C), at pH 5.5-8.5 (optimum pH 7.0) and in the presence of 0-35 g NaCl l(-1) (optimum 1 g NaCl l(-1)). It fermented yeast extract and a wide range of carbohydrates including cellobiose, D-glucose, D-ribose, sucrose, D-xylose, maltose, D-galactose and starch as electron donors. Acetate, ethanol, CO2 and H-2 were end products of glucose metabolism. It reduced elemental sulfur, but not sulfate, thiosulfate or sulfite, into sulfide. The DNA G+C content was 37.6 mol%. The predominant cellular fatty acids were C-14 : 0 and C-16 : 0. Phylogenetic analysis of the 16S rRNA gene sequence suggested Fusibacter bizertensis as the closest relative of this isolate (identity of 97.2 % to the type strain). Based on phenotypic, phylogenetic and genotypic taxonomic characteristics, strain KhalAKB1(T) is proposed to be assigned to a novel species within the genus Fusibacter, order Clostridiales, Fusibacter fontis sp. nov. The type strain is KhalAKB1(T) (=DSM 28450(T)=JCM 19912(T)). %$ 084