%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Cordeiro, L. D.
%A Simoes-Gurgel, C.
%A Albarello, N.
%A Engelmann, Florent
%T Cryopreservation of in vitro-grown shoot tips of Cleome rosea Vahl (Cleomaceae) using the V cryo-plate technique
%D 2015
%L fdi:010065477
%G ENG
%J In Vitro Cellular and Developmental Biology-Plant
%@ 1054-5476
%K Aluminum cryo-plates ; 6-Benzyladenine ; Germplasmconservation ; Medicinal plant ; Restingas
%M ISI:000365274200011
%N 6
%P 688-695
%R 10.1007/s11627-015-9714-9
%U https://www.documentation.ird.fr/hor/fdi:010065477
%> https://www.documentation.ird.fr/intranet/publi/2015/12/010065477.pdf
%V 51
%W Horizon (IRD)
%X This report highlights the first successful cryopreservation of in vitro shoot tips of Cleome rosea, achieved by the vitrification technique using aluminum cryo-plates (V cryo-plate). The effects on survival and recovery of C. rosea shoot tips of different plant vitrification solutions (PVS2 and PVS3), light intensity (standard and dim light), and 6-benzyladenine (BA) supplementation (0.10, 0.25, or 0.50 mg L-1 for 1 or 3 wk) in the recovery medium were investigated. Cryopreserved shoot tips showed high regeneration frequencies when treated with PVS2 and PVS3, reaching survival frequencies of 97 and 70%, respectively. When placed onto medium without growth regulators, recovery of cryopreserved shoot tips did not exceed 33% (obtained with PVS2) or 23% (PVS3). Supplementation of recovery medium with 0.5 mg L-1 BA for 3 wk increased both survival and recovery to 100% after cryopreservation following treatment with either PVS2 or PVS3. These values were maintained even when shoot tips were cultured in the presence of 0.10 mg L-1 BA for 1 wk. Maintenance under dim light resulted in better phenotypic characteristics of plants produced from cryopreserved shoot tips.
%$ 076 ; 084