@article{fdi:010065477,
  title = {{C}ryopreservation of in vitro-grown shoot tips of {C}leome rosea {V}ahl ({C}leomaceae) using the {V} cryo-plate technique},
  author = {{C}ordeiro, {L}. {D}. and {S}imoes-{G}urgel, {C}. and {A}lbarello, {N}. and {E}ngelmann, {F}lorent},
  editor = {},
  language = {{ENG}},
  abstract = {{T}his report highlights the first successful cryopreservation of in vitro shoot tips of {C}leome rosea, achieved by the vitrification technique using aluminum cryo-plates ({V} cryo-plate). {T}he effects on survival and recovery of {C}. rosea shoot tips of different plant vitrification solutions ({PVS}2 and {PVS}3), light intensity (standard and dim light), and 6-benzyladenine ({BA}) supplementation (0.10, 0.25, or 0.50 mg {L}-1 for 1 or 3 wk) in the recovery medium were investigated. {C}ryopreserved shoot tips showed high regeneration frequencies when treated with {PVS}2 and {PVS}3, reaching survival frequencies of 97 and 70%, respectively. {W}hen placed onto medium without growth regulators, recovery of cryopreserved shoot tips did not exceed 33% (obtained with {PVS}2) or 23% ({PVS}3). {S}upplementation of recovery medium with 0.5 mg {L}-1 {BA} for 3 wk increased both survival and recovery to 100% after cryopreservation following treatment with either {PVS}2 or {PVS}3. {T}hese values were maintained even when shoot tips were cultured in the presence of 0.10 mg {L}-1 {BA} for 1 wk. {M}aintenance under dim light resulted in better phenotypic characteristics of plants produced from cryopreserved shoot tips.},
  keywords = {{A}luminum cryo-plates ; 6-{B}enzyladenine ; {G}ermplasmconservation ; {M}edicinal plant ; {R}estingas},
  booktitle = {},
  journal = {{I}n {V}itro {C}ellular and {D}evelopmental {B}iology-{P}lant},
  volume = {51},
  numero = {6},
  pages = {688--695},
  ISSN = {1054-5476},
  year = {2015},
  DOI = {10.1007/s11627-015-9714-9},
  URL = {https://www.documentation.ird.fr/hor/fdi:010065477},
}