@article{fdi:010065468,
  title = {{D}iscovery of genome-wide microsatellite markers in {S}combridae : a pilot study on albacore tuna},
  author = {{N}ikolic, {N}. and {D}uthoy, {S}. and {D}estombes, {A}. and {B}odin, {N}athalie and {W}est, {W}. and {P}uech, {A}. and {B}ourjea, {J}.},
  editor = {},
  language = {{ENG}},
  abstract = {{R}ecent developments in sequencing technologies and bioinformatics analysis provide a greater amount of {DNA} sequencing reads at a low cost. {M}icrosatellites are the markers of choice for a variety of population genetic studies, and high quality markers can be discovered in non-model organisms, such as tuna, with these recent developments. {H}ere, we use a high-throughput method to isolate microsatellite markers in albacore tuna, {T}hunnus alalunga, based on coupling multiplex enrichment and next-generation sequencing on 454 {GS}-{FLX} {T}itanium pyrosequencing. {T}he crucial minimum number of polymorphic markers to infer evolutionary and ecological processes for this species has been described for the first time. {W}e provide 1670 microsatellite design primer pairs, and technical and molecular genetics selection resulting in 43 polymorphic microsatellite markers. {O}n this panel, we characterized 34 random and selectively neutralmarkers ("neutral") and 9 "non-neutral" markers. {T}he variability of "neutral" markers was screened with 136 individuals of albacore tuna from southwest {I}ndian {O}cean (42), northwest {I}ndian {O}cean (31), {S}outh {A}frica (31), and southeast {A}tlantic {O}cean (32). {P}ower analysis demonstrated that the panel of genetic markers can be applied in diversity and population genetics studies. {G}lobal genetic diversity for albacore was high with a mean number of alleles at 16.94; observed heterozygosity 66% and expected heterozygosity 77%. {T}he number of individuals was insufficient to provide accurate results on differentiation. {O}f the 9 "non-neutral" markers, 3 were linked to a sequence of known function. {T}he one is located to a sequence having an immunity function ({T}hu{A}la-{T}cell-01) and the other to a sequence having energy allocation function ({T}hu{A}la-{H}ki-01). {T}hese two markers were genotyped on the 136 individuals and presented different diversity levels. {T}hu{A}la-{T}cell-01 has a high number of alleles (20), heterozygosity (87-90%), and assignment index. {T}hu{A}la-{H}ki-01 has a lower number of alleles (9), low heterozygosity (24-27%), low assignment index and significant inbreeding. {F}inally, the 34 "neutral" and 3 "non-neutral" microsatellites markers were tested on four economically important {S}combridae species-{T}hunnus albacares, {T}hunnus thynnus, {T}hunnus obesus, and {A}canthocybium solandri.},
  keywords = {{OCEAN} {INDIEN} {OUEST} ; {ATLANTIQUE} {SUD} {EST} ; {AFRIQUE} {DU} {SUD} ; {SEYCHELLES}},
  booktitle = {},
  journal = {{P}los {O}ne},
  volume = {10},
  numero = {11},
  pages = {e0141830 [22 p.]},
  ISSN = {1932-6203},
  year = {2015},
  DOI = {10.1371/journal.pone.0141830},
  URL = {https://www.documentation.ird.fr/hor/fdi:010065468},
}