Publications des scientifiques de l'IRD

Chauvin P., Menard S., Iriart X., Nsango S. E., Tchioffo M. T., Abate Luc, Awono-Ambene P. H., Morlais Isabelle, Berry A. (2015). Prevalence of Plasmodium falciparum parasites resistant to sulfadoxine/pyrimethamine in pregnant women in Yaounde, Cameroon : emergence of highly resistant pfdhfr/pfdhps alleles. Journal of Antimicrobial Chemotherapy, 70 (9), p. 2566-2571. ISSN 0305-7453.

Titre du document
Prevalence of Plasmodium falciparum parasites resistant to sulfadoxine/pyrimethamine in pregnant women in Yaounde, Cameroon : emergence of highly resistant pfdhfr/pfdhps alleles
Année de publication
2015
Type de document
Article référencé dans le Web of Science WOS:000363248300021
Auteurs
Chauvin P., Menard S., Iriart X., Nsango S. E., Tchioffo M. T., Abate Luc, Awono-Ambene P. H., Morlais Isabelle, Berry A.
Source
Journal of Antimicrobial Chemotherapy, 2015, 70 (9), p. 2566-2571 ISSN 0305-7453
Objectives: To determine, 6 years after the adoption of intermittent preventive treatment of pregnant women with sulfadoxine/pyrimethamine (IPTp-SP) in Cameroon, (i) the polymorphism and prevalence of Plasmodium falciparum dihydrofolate reductase (pfdhfr) and dihydropteroate synthase (pfdhps) gene mutations associated with sulfadoxine/pyrimethamine resistance and (ii) the consequences of sulfadoxine/pyrimethamine use in the selection of pfdhfr/pfdhps alleles. Methods: pfdhfr and pfdhps genes from P. falciparum isolates collected in Yaounde (Cameroon) from pregnant women with symptomatic malaria before taking IPTp-SP [SP2 group (control) (n = 51)] or afterwards [SP+ group (n = 49)] were sequenced. Results: The pfdhfr N51I, C59R, S108N triple mutant had a prevalence close to 100% (96/100) and no mutations at codons 50 and 164 were detected in either of the groups. The most frequent pfdhps mutation was A437G with a prevalence of 76.5% (39/51) in the SP2 group, which was significantly higher in pregnant women who took sulfadoxine/pyrimethamine [95.9% (47/49)] (P = 0.012). Our study confirmed the presence of the pfdhps K540E mutation in Cameroon, but it remained rare. The prevalence of pfdhps A581G and A613S mutations had increased [5.9% (3/51) and 11.8% (6/51) in the control group, respectively] since the last studies in 2005. Surprisingly, the new pfdhps I431V mutation was detected, at a prevalence of 9.8% (5/51), and was found to be associated with other pfdhfr/pfdhps alleles to form an octuple N51I, C59R, S108N/I431V, S436A, A437G, A581G, A613S mutant. Conclusions: Significant changes were found in pfdhps polymorphism. In particular, we observed several parasites carrying eight mutations in pfdhfr/pfdhps genes, which are very susceptible to having a high level of resistance to sulfadoxine/pyrimethamine.
Plan de classement
Santé : généralités [050] ; Entomologie médicale / Parasitologie / Virologie [052] ; Urbanisation et sociétés urbaines [102]
Description Géographique
CAMEROUN ; YAOUNDE
Localisation
Fonds IRD [F B010065397]
Identifiant IRD
fdi:010065397
Contact