@article{fdi:010065377,
  title = {{A}n. gambiae g{SG}6-{P}1 evaluation as a proxy for human-vector contact in the {A}mericas : a pilot study},
  author = {{L}ondono-{R}enteria, {B}. and {D}rame, {P}. {M}. and {W}eitzel, {T}. and {R}osas, {R}. and {G}ripping, {C}. and {C}ardenas, {J}. {C}. and {A}lvares, {M}. and {W}esson, {D}. {M}. and {P}oinsignon, {A}nne and {R}emou{\'e}, {F}ranck and {C}olpitts, {T}. {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {D}uring blood meal, the female mosquito injects saliva able to elicit an immune response in the vertebrate. {T}his immune response has been proven to reflect the intensity of exposure to mosquito bites and risk of infection for vector transmitted pathogens such as malaria. {T}he peptide g{SG}6-{P}1 of {A}n. gambiae saliva has been demonstrated to be antigenic and highly specific to {A}nopheles as a genus. {H}owever, the applicability of g{SG}6-{P}1 to measure exposure to different {A}nopheles species endemic in the {A}mericas has yet to be evaluated. {T}he purpose of this pilot study was to test whether human participants living in {A}merican countries present antibodies able to recognize the g{SG}6-{P}1, and whether these antibodies are useful as a proxy for mosquito bite exposure and malaria risk. {M}ethods: {W}e tested human serum samples from {C}olombia, {C}hile, and the {U}nited {S}tates for the presence of {I}g{G} antibodies against g{SG}6-{P}1 by {ELISA}. {A}ntibody concentrations were expressed as delta optical density ({D}elta {OD}) of each sera tested in duplicates. {T}he difference in the antibody concentrations between groups was tested using the nonparametric {M}ann {W}hitney test (independent groups) and the nonparametric {W}ilcoxon matched-pairs signed rank test (dependent groups). {A}ll differences were considered significant with a {P} < 0.05. {R}esults: {W}e found that the concentration of g{SG}6-{P}1 antibodies was significantly correlated with malaria infection status and mosquito bite exposure history. {P}eople with clinical malaria presented significantly higher concentrations of {I}g{G} anti-g{SG}6-{P}1 antibodies than healthy controls. {A}dditionally, a significant raise in antibody concentrations was observed in subjects returning from malaria endemic areas. {C}onclusion: {O}ur data shows that g{SG}6-{P}1 is a suitable candidate for the evaluation of exposure to {A}nopheles mosquito bites, risk of malaria transmission, and effectiveness of protection measures against mosquito bites in the {A}mericas.},
  keywords = {{CHILI} ; {COLOMBIE} ; {ETATS} {UNIS}},
  booktitle = {},
  journal = {{P}arasites and {V}ectors},
  volume = {8},
  numero = {},
  pages = {art. 533 [8 p.]},
  ISSN = {1756-3305},
  year = {2015},
  DOI = {10.1186/s13071-015-1160-3},
  URL = {https://www.documentation.ird.fr/hor/fdi:010065377},
}