@article{fdi:010065339,
  title = {{E}ffects of earthworms on the fungal community and microbial activity in root-adhering soil of {L}antana camara during phytoextraction of lead},
  author = {{J}usselme, {M}. {D}. and {P}oly, {F}. and {L}ebeau, {T}. and {R}ouland {L}ef{\`e}vre, {C}orinne and {M}iambi, {E}.},
  editor = {},
  language = {{ENG}},
  abstract = {{E}arthworms are known to stimulate soil microorganisms and to enhance phytoextraction of metals. {T}his study was designed to test the hypothesis that the positive effect of earthworms on phytoextraction performance results from the stimulation of soil fungi that are known to be tolerant to heavy metals in contaminated soils. {I}t set out to assess the relationships between lead phytoextraction and the changes in soil fungi in response to earthworm activity. {T}he experiment was performed in outdoor microcosms combining: earthworms ({P}ontoscolex corethrurus), {S}panish {F}lag ({L}antana camara) and soil spiked with 500 and 1000 mg {P}b kg(-1). {C}ultivable fungi were enumerated using plate counts. {PCR}-{DGGE} analysis targeting the 28{S} r{RNA} gene was used to determine the structure of the fungal community. {T}otal microbial activity was measured by fluorescein diacetate ({FDA}) hydrolysis assay. {P}lant {P}b uptake by {L}. camara increased by about 2.5-fold in the presence of earthworms, regardless of the {P}b concentration. {T}his was significantly correlated with the increase in total microbial activity, abundance of cultivable fungi and richness index of the fungal community calculated from {DGGE} banding patterns. {I}n the presence of earthworms, there was a significant positive correlation between microbial activity, plant biomass and plant {P}b uptake. {T}here was no correlation for the control without earthworms. {T}hese results provide evidence that interactions between earthworms and soil microorganisms have a positive effect on {P}b-phytoextraction yield. {T}he study of the ecological context of phytoremediation should be broadened by considering the interactions between plants, microorganisms and earthworms that affect heavy metal uptake by plants.},
  keywords = {{E}arthworm ; {F}ungal community ; {M}icrobial activity ; {H}eavy metals ; {L}antana camara ; {P}hytoextraction},
  booktitle = {},
  journal = {{A}pplied {S}oil {E}cology},
  volume = {96},
  numero = {},
  pages = {151--158},
  ISSN = {0929-1393},
  year = {2015},
  DOI = {10.1016/j.apsoil.2015.07.011},
  URL = {https://www.documentation.ird.fr/hor/fdi:010065339},
}